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Virologica Sinica, 23 (3) : 203-210, 2008
Research Article
Isolation and Identification of Canine Parvovirus Serotype 2a and Its VP2 Protein Expression in Transgenic Tobacco
.State Key Laboratory of Virology, Wuhan Institute of Virology, the Chinese Academy of Sciences, Wuhan 430071, China
2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
A strain of canine parvovirus(CPV)was isolated from feces of an ill puppy in an animal hospital in Wuhan, China. It was designated as CPV/WH02/06. This isolate was identified as serotype CPV-2a by the hemagglutination test, CPV Ag detection strip, electron microscopy, and PCR. The vp2 gene was cloned and sequenced and assigned GenBank accession number EU377537. A 1242 bp segment of the 5’ region of the vp2 gene was cloned and inserted into the binary vector pBI121 and used for Agrobacterium-mediated tobacco transformation. Transgenic tobacco plants were selected on MS medium supplemented with 100 μg/mL kanamycin and 100 μg/mL timentin. Integration of the vp2 gene into the tobacco genome was confirmed by PCR using T1 progeny plants, and the expression of the VP2 protein was confirmed by Western blotting.
Key Words: Canine parvovirus;  Isolation;  VP2;  Tobacco;  Expression
  Published online: 5 Jun 2008
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