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Virologica Sinica, 23 (3) : 167-172, 2008
One New Method of Nucleic Acid Amplification —Loop-mediated Isothermal Amplification of DNA
1. School of Life Science, Shanghai University, Shanghai 200444, China
2. Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.
  Published online: 5 Jun 2008
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