Hello guest, Register  |  Sign In
Virologica Sinica, 23 (4) : 233-246, 2008
Research Article
Comparison of the Immunogenicities of HIV-1 Mutants Based on Structural Modification of env
1.Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, No. 2 Tiantanxili, Beijing 100050, China
2. College of Life Science, Jilin University, Changchun 130012, China
3. Aaron Diamond AIDS Research Center, The Rockefeller University, 455 First Avenue, New York, NY 10016,USA
 Correspondence:
(436.55KB)  
Abstract
Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.
  Published online: 5 Aug 2008
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved