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Virologica Sinica, 23 (4) : 233-246, 2008
Research Article
Comparison of the Immunogenicities of HIV-1 Mutants Based on Structural Modification of env
1.Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, No. 2 Tiantanxili, Beijing 100050, China
2. College of Life Science, Jilin University, Changchun 130012, China
3. Aaron Diamond AIDS Research Center, The Rockefeller University, 455 First Avenue, New York, NY 10016,USA
Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.
  Published online: 5 Aug 2008
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