1. State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention IVDC, China CDC, Beijing 100052, China 2. Department of Genetic Engineering, Wuhan Institute of Biological Products, Wuhan 430060, China 3. Laboratory of Epidemiology, Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, China
To obtain the helper plasmids for a reverse genetics system of rabies virus, the cDNAs of the complete open reading frames of the N, P, G, and L genes of rabies street virus stain HN10 were each cloned into expression vector pVAX1, These four plasmids were identified by restriction enzyme digestion and gene sequencing. The plasmid encoding the N protein was selected to determine the expression effect of these plasmids in NA cells. The results showed that the helper plasmids for a reverse genetics system of rabies street virus strain HN10 had been successfully constructed.