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Virologica Sinica, 24 (6) : 573-578, 2009
Research Article
Confirmed Diagnosis by RT-PCR and Phylogenetic Analysis of Peste des Petits Ruminants Viruses in Tibet, China*
1. Laboratory of Tropical and Subtropical Animal Virology of MOA, National Exotic Animal Disease Diagnostic Laboratory, Kunming 650224, China
2. Yu Xi Agriculture Vocational Technical College, Yu Xi 653100, China
3. Peking Union Medical College, Tsinghua University, Kunming Jiaoling Road 379#,Kunming 650118,China
 Correspondence: yangsb3799@sina.com
(456.29KB)  
Abstract

This paper reports the confirmed diagnosis by nested RT-PCR of PPR cases in Tibet, China in 2007, and results of phylogenetic analysis. Results showed that the 11 tested samples were PPRV positive by nested RT-PCR, of which 2 samples were genetically close to the X7443 strain (Nigeria 75/1) of lineage I, and 3 samples close to the strain AY560591 (Sungri96) of linage IV with 96.6%、97.3%、97.6% and 98% nucleotide sequence homogeneity respectively, based on partial sequencing of the F gene from 5 samples and complete sequencing of the N/M/F/H genes from one sample. This study suggested that there are at least 2 origins of PPRV in China.

Received: 6 Jul 2009  Accepted: 21 Apr 2009  Published online: 5 Dec 2009
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