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Virologica Sinica, 25 (3) : 168-176, 2010
Establishment of an ELISA to Detect Kaposi’s Sarcoma-associated Herpesvirus Using Recombinant ORF73
1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
2. Department of Microbiology, Anhui Medical University, Hefei 230032, China
3. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
4. Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University, Shihezi 832002, China
5. Shanghai Institute of Biological Products, Shanghai 200052, China
Kaposi’s sarcoma-associated herpesvirus (KSHV) is causally related to Kaposi’s sarcoma (KS), primary effusion lymphoma (PEL) and a proportion of cases of multicentric Castleman’s disease (MCD). The ORF73 protein was cloned into pQE80L-orf73 and expressed in E.coli and purified. The expressed recombinant ORF73 was identified by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). A protein of about 27 kDa was expressed as expected. Western Blotting showed that the purified recombinant ORF73 reacted with KSHV positive serum. The immunogenicity of the recombinant ORF73 was further analysed by ELISA and the optimal conditions were determined. The ORF73 ELISA was used to compare the KSHV seroprevalence between Hubei and Xinjiang Han people. The Han people in Xinjiang have significantly higher KSHV seroprevalence than their counterparts in Hubei (6.7% vs 2.9%, P = 0.005).
  Published online: 5 Jun 2010
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