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Virologica Sinica, 26 (5) : 324-337, 2011
Evaluation of Efficacy of Stabilizers on the Thermostability of Live Attenuated Thermo-adapted Peste des petits ruminants Vaccines
1 Division of Virology, Indian Veterinary Research Institute, Campus Mukteswar 263138, Nainital District, Uttarakhand, India
2 Veterinary Type cultures Centre, National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India
3 Project Directorate on Animal Disease Monitoring And Surveillance PD-ADMAS, Hebbal, Bangalore 560024, India
4 National Research Centre on Equines, Sirsa Road, Hisar 125001, Haryana, India
 Correspondence: rks_virology@rediffmail.com
In this study, thermo-adapted (Ta) PPR vaccines were assessed for their stability at 25, 37, 40, 42 and 45℃ in lyophilized form using two extrinsic stabilizers {lactalbumin hydrolysate-sucrose (LS) and stabilizer E} and in reconstituted form with the diluents (1 mol/L MgSO4 or 0.85% NaCl). The lyophilized vaccines showed an expiry period of 24-26 days at 25℃, 7-8 days at 37℃ and 3-4 days at 40℃. LS stabilizer was superior at 42℃ with a shelf-life of 44 h, whereas in stabilizer E, a 40 h shelf-life with a comparable half-life was observed. At 45℃, the half-life in stabilizer E was better than LS and lasted for 1 day. Furthermore, the reconstituted vaccine maintained the titre for 48 h both at 4℃ and 25℃ and for 24-30 h at 37℃. As both the stabilizers performed equally well with regard to shelf-life and half-life, the present study suggests LS as stabilizer as a choice for lyophilization with 0.85% NaCl diluent, because it has better performance at higher temperature. These Ta vaccines can be used as alternatives to existing vaccines for the control of the disease in tropical countries as they are effective in avoiding vaccination failure due to the breakdown in cold-chain maintenance, as this vaccine is considerably more stable at ambient temperatures.
  Published online: 5 Oct 2011
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