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Virologica Sinica, 26 (5) : 338-346, 2011
Production of CCHF Virus-Like Particle by a Baculovirus-Insect Cell Expression System*
Zhao-rui Zhou, Man-li Wang**, Fei Deng, Tian-xian Li, Zhi-hong Hu and Hua-lin Wang
 Correspondence: Wangml@wh.iov.cn
Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).
  Published online: 5 Oct 2011
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