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Virologica Sinica, 26 (6) : 403-408, 2011
Bovine Herpesvirus 1 Protein bICP0 Represses the Transcription of bISG15 in Fetal Bovine Lung Cells*
1. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education
Key Laboratory of Microbial Functional Genomics Tianjin
Nankai University, Tianjin 300071, China
2. School of Medicine, Nankai University, Tianjin 300071, China
 Correspondence: gengyq@nankai.edu.cn
The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells. Bovine Herpesvirus 1(BHV-1), which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-1’s infection could repress the basal or inducible transcription of bISG15 in FBL cells. It demonstrates that this repression effect depends on BHV-1 viral infection and new protein synthesis. Our previous work showed that bIRF-3 was the key factor in the stimulation of bISG15 in FBL cells, so the effect of BHV-1 viral protein on bIRF-3 activating the promoter of bISG15 was confirmed. The luciferase assay showed the BHV-1 viral protein bICP0 inhibited the activation of bISG15 promoter stimulated by bIRF-3. Taken together, our work suggested that BHV-1 had some molecular mechanism to resist the cellular bISG15’s antiviral functions.
Received: 27 Oct 2011  Accepted: 21 Jul 2011  Published online: 5 Dec 2011
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