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Virologica Sinica, 27 (2) : 93-99, 2012
Characterization of Pigeon Paramyxoviruses (Newcastle disease virus) Isolated in Kazakhstan in 2005*
1.Institute of Microbiology & Virology, Almaty 050010, Kazakhstan
2.D.I. Ivanovsky Institute of Virology, Russian Academy of medical sciences, Moscow 123098, Russia
 Correspondence: anpav_63@mail.ru
Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the amplified F protein DNA product (nucleotide sequence 47–422) and the deduced amino acid sequences were compared phylogenetically with those from strains previously reported in other geographic regions. Phylogenetic analysis indicated that the Kazakhstanian pigeon paramyxovirus type 1 (PPMV-1) isolates belong to genotype VI or 4bii. To our knowledge, this is the first reported VI isolates that possess the sequences of 112 GKRQKR116* F117 within the F0 protein. The information is fundamental to improving the efficiency of control strategies and vaccine development for NDV.
Received: 27 Feb 2012  Accepted: 18 Nov 2011  Published online: 5 Apr 2012
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