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Virologica Sinica, 27 (3) : 172-178, 2012
Preparation and Identification of Anti-rabies Virus Monoclonal Antibodies*
1. College of Animal Science and Veterinary Medicine, Qingdao Agricultural university, Qingdao 266109, China
2. State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
3. Xinjiang Center for Disease Control and Prevention, Xinjiang 830001, China
 Correspondence: qtang04@sina.com
(445.79KB)  
Abstract
To provide a foundation for the development of rapid and specific methods for the diagnosis of rabies virus infection, anti-rabies virus monoclonal antibodies were prepared and rabies virus nucleoprotein and human rabies virus vaccine strain (PV strain) were used as immunogens to immunize 6-8 week old female BALB/c mice. Spleen cells and SP2/0 myeloma cells were fused according to conventional methods: the monoclonal cell strains obtained were selected using the indirect immunofluorescence test; this was followed by preparation of monoclonal antibody ascitic fluid; and finally, systematic identification of subclass, specificity and sensitivity was carried out. Two high potency and specific monoclonal antibodies against rabies virus were obtained and named 3B12 and 4A12, with ascitic fluid titers of 1:8000 and 1:10000, respectively. Both belonged to the IgG2a subclass. These strains secrete potent, stable and specific anti-rabies virus monoclonal antibodies, which makes them well suited for the development of rabies diagnosis reagents.
Received: 28 Mar 2012  Accepted: 8 Feb 2012  Published online: 5 Jun 2012
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