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Virologica Sinica, 27 (4) : 254-258, 2012
Research Article
Expression and Purification of Enterovirus Type 71 Polyprotein P1 using Pichia pastoris system
College of life science, Jianghan University, Wuhan 430056, Hubei, China
 Correspondence: hanxue_whdx@163.com
(478.84KB)  
Abstract

Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease (HFMD) resulting in hundreds of deaths of children every year; However, currently, there is no effective treatment for EV71. In this study, the EV71 poly-protein (EV71-P1 protein) gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GS115. The EV71 P1 protein with a molecular weight of 100 kD was produced and secreted into the medium. The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70%. The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits. We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.

Received: 3 Jul 2012  Accepted: 24 Apr 2012  Published online: 5 Aug 2012
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