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Virologica Sinica, 28 (1) : 36-42, 2013
Research Article
An Inactivated Vaccine from a Field Strain of Bovine Herpesvirus-1 (BoHV-1) has High Antigenic Mass and Induces Strong Efficacy in a Rabbit Model
1. Microbiology and Epidemiology Group, Faculty of Veterinary Medicine and Animal Science, National University of Colombia, Bogotá
11001, Colombia

2. Grupo de Investigació
n CENTAURO, Agrarian Sciences Faculty, Universidad de Antioquia, A.A. 1226, Medellí
n, Colombia
 Correspondence: julianruizsaenz@gmail.com
(443.80KB)  
Abstract
Bovine Herpesvirus-1 (BoHV-1) is a DNA virus belonging to the family Herpesviridae, subfamily Alfaherpesvirinae; it is a worldwide pathogen, causing serious economic losses in livestock. In Colombia there have been multiple isolates of BoHV-1 that have been subjected to molecular characterization, classifying most of the country isolates as BoHV-1.1. In the present study we developed and evaluated an ethyleneimine binary inactivated isolate from the native BoHV-1 strain (Córdoba-2) in a rabbit model of vaccination and infection. The vaccine was evaluated in two phases, one of immunogenicity with vaccination and a booster after 21 days, and an evaluation phase of protection against challenge with a highly virulent reference strain. The results demonstrate optimum serum-conversion, with protective neutralizing antibody titers 28 days post vaccination and optimal protection against challenge with the reference strain with decreased clinical signs of infection, protection against the onset of fever and decrease of virus excretion post challenge. In conclusion, our results show the enormous potential that an immunogenic inactivated vaccine has produced from the native BoHV-1.1 strain, which produces a high antigen mass to the vaccine to induce optimal immunity and protection, and it is a strong candidate for evaluation and possible future use in different cattle populations.
Received: 17 Jan 2013  Accepted: 7 Sep 2012  Published online: 1 Feb 2013
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