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Virologica Sinica, 28 (2) : 92-96, 2013
Research Article
Identification of Immunogenic Determinants of the Spike Protein of SARS-like Coronavirus
1. Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences CAS, Wuhan 430071, China

2. CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong 3220, Australia

3. Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore 169857
 Correspondence: zlshi@wh.iov.cn
(451.51KB)  
Abstract
Bat SARS-like coronavirus (SL-CoV) has a genome organization almost identical to that of SARS-CoV, but the N-terminus of the Spike (S) proteins, which interacts with host receptor and is a major target of neutralizing antibodies against CoVs, of the two viruses has only 63-64% sequence identity. Although there have been reports studying the overall immunogenicity of SSL, knowledge on the precise location of immunodominant determinants for SSL is still lacking. In this study, using a series of truncated expressed SSL fragments and SSL specific mouse sera, we identified two immunogenic determinants for SSL. Importantly, one of the two regions seems to be located in a region not shared by known immunogenic determinants of the SSARS. This finding will be of potential use in future monitoring of SL-CoV infection in bats and spillover animals and in development of more effective vaccine to cover broad protection against this new group of coronaviruses.
Key Words: Bat;  SL-CoV;  Immune;  Vaccine
  Accepted: 8 Nov 2012  Published online: 5 Apr 2013
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