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Virologica Sinica, 28 (5) : 266-271, 2013
Research Article
Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
 Correspondence: zhangqy@ihb.ac.cn
(524.20KB)  
Abstract
The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa, was isolated from Lake Dianchi,Kunming,China. Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC. Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages,and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis. Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length. Using one-step growth experiments,the latent period and burst size of MaMV-DC were estimated to be 24-48 hours and approximately 80 infectious units per cell,respectively. Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA,and the genome size was estimated to be approximately 160 kb. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed four major structural proteins. These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.
Received: 26 Aug 2013  Accepted: 3 May 2013  Published online: 3 Oct 2013
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