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Virologica Sinica, 29 (2) : 94-102, 2014
Research Article
Characterization of a full-length infectious clone of bovine foamy virus 3026
1. Key Laboratory of Molecular Microbiology and Biotechnology Ministry of Education and Key Laboratory of Microbial Functional Genomics Tianjin, College of Life Sciences, Nankai University, Tianjin 300071, China
2. Department of Molecular and Cellular Pharmacology, the Vascular Biology Institute, University of Miami School of Medicine, Miami, FL 33136, USA
3. National Laboratory of Biomacromolecules, Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
2. Department of Molecular and Cellular Pharmacology, the Vascular Biology Institute, University of Miami School of Medicine, Miami, FL 33136, USA
3. National Laboratory of Biomacromolecules, Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Correspondence: wentaoqiao@nankai.edu.cn
(1237.87KB) 
Abstract
The biological features of most foamy viruses (FVs) are poorly understood, including bovine foamy virus (BFV). BFV strain 3026 (BFV3026) was isolated from the peripheral blood mononuclear cells of an infected cow in Zhangjiakou, China. A full-length genomic clone of BFV3026 was obtained from BFV3026-infected cells, and it exhibited more than 99% amino acid (AA) homology to another BFV strain isolated in the USA. Upon transfection into fetal canine thymus cells, the full-length BFV3026 clone produced viral structural and auxiliary proteins, typical cytopathic effects, and virus particles. These results demonstrate that the full-length BFV3026 clone is fully infectious and can be used in further BFV3026 research.
Received: 3 Mar 2014 Accepted: 26 Sep 2013
Published online: 1 Apr 2014
