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Virologica Sinica, 29 (4) : 199-210, 2014
Research Article
Labeling of influenza viruses with synthetic fluorescent and biotin-labeled lipids
1. Food and Drug Administration, 29 Lincoln Drive, Bethesda 20892, MD, USA

2. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya 16/10, Moscow 117997, Russia

3. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region 142782, Russia

4. Biotechnology Research Institute, AUT University, Private Bag 92006, Auckland 1142, New Zealand
 Correspondence: natalia.ilyushina@fda.hhs.gov;bovin@carb.ibch.ru
(1817.50KB)  
Abstract
Direct labeling of virus particles is a powerful tool for the visualization of virus-cell interaction events. However, this technique involves the chemical modification of viral proteins that affects viral biological properties. Here we describe an alternative approach of influenza virus labeling that utilizes Function-Spacer-Lipid (FSL) constructs that can be gently inserted into the virus membrane. We assessed whether labeling with fluorescent (fluo-Ad-DOPE) or biotin-labeled (biot-CMG2-DOPE) probes has any deleterious effect on influenza virus hemagglutinin (HA) receptor specificity, neuraminidase (NA) activity, or replicative ability in vitro. Our data clearly show that neither construct significantly affected influenza virus infectivity or viral affinity to sialyl receptors. Neither construct influenced the NA activities of the influenza viruses tested, except the A/Puerto Rico/8/34 (H1N1) strain. Our data indicate that lipid labeling provides a powerful tool to analyze influenza virus infection in vitro.
Received: 31 Jul 2014  Accepted: 18 May 2014  Published online: 1 Aug 2014
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