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Virologica Sinica, 29 (4) : 228-236, 2014
Research Article
A panel of monoclonal antibodies against the prion protein proves that there is no prion protein in human pancreatic ductal epithelial cells
1. State Key Laboratory of Virology and Department of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

2. Department of Virology, School of Life Sciences, Wuhan University, Wuhan 430072, China

3. Department of Molecular Endocrinology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

4. Department of Pathology, Case Western Reserve University, Cleveland, OH 44124, USA
 Correspondence: cyli@wh.iov.cn
Prion diseases are a group of neurodegenerative diseases that are fatal. The study of these unique diseases in China is hampered by a lack of resources. Amongst the most important resources for biological study are monoclonal antibodies. Here, we characterize a panel of monoclonal antibodies specific for cellular prion protein by enzyme-linked immunosorbent assay (ELISA), immunofluorescent staining, flow cytometry, and western blotting. We identify several antibodies that can be used for specific applications and we demonstrate that there is no prion protein expression in human pancreatic ductal epithelial cells (HPDC).
Received: 14 Aug 2014  Accepted: 29 May 2014  Published online: 1 Aug 2014
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