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Virologica Sinica, 29 (6) : 381-392, 2014
Research Article
Binding of HIV-1 virions to α4β7 expressing cells and impact of antagonizing α4β7 on HIV-1 infection of primary CD4+ T cells
1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

2. University of Chinese Academy of Sciences, Beijing 100049, China

3. Department of General Surgery, Wuhan No.1 Hospital, Wuhan 430022, China

4. Section of Infectious Diseases, Faculty of Medicine, Imperial College London, St. Mary's Campus, London W21PG, UK

5. Institute for Infection and Immunity, St George's University of London, London SW170RE, UK
 Correspondence: qhu@wh.iov.cn
(3392.07KB)  
Abstract
HIV-1 envelope glycoprotein is reported to interact with α4β7, an integrin mediating the homing of lymphocytes to gut-associated lymphoid tissue, but the signifi cance of α4β7 in HIV-1 infection remains controversial. Here, using HIV-1 strain BaL, the gp120 of which was previously shown to be capable of interacting with α4β7, we demonstrated that α4β7 can mediate the binding of whole HIV-1 virions to α4β7-expressing transfectants. We further constructed a cell line stably expressing α4β7 and confirmed the α4β7-mediated HIV-1 binding. In primary lymphocytes with activated α4β7 expression, we also observed significant virus binding which can be inhibited by an anti-α4β7 antibody. Moreover, we investigated the impact of antagonizing α4β7 on HIV-1 infection of primary CD4+ T cells. In α4β7-activated CD4+ T cells, both anti-α4β7 antibodies and introduction of shorthairpin RNAs specifically targeting α4β7 resulted in a decreased HIV-1 infection. Our findings indicate that α4β7 may serve as an attachment factor at least for some HIV-1 strains. The established approach provides a promising means for the investigation of other viral strains to understand the potential roles of α4β7 in HIV-1 infection.
Received: 10 Dec 2014  Accepted: 21 Oct 2014  Published online: 1 Dec 2014
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