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Virologica Sinica, 30 (1) : 52-58, 2015
Research Article
Isolation and characterization of glacier VMY22, a novel lytic cold-active bacteriophage of Bacillus cereus
1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China

2. Faculty of Life Science, Wuhan University, Wuhan 430072, China
 Correspondence: weiyunlin18@gmail.com
(3749.61KB)  
Abstract
As a unique ecological system with low temperature and low nutrient levels, glaciers are considered a “living fossil” for the research of evolution. In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier in China, and its characteristics were studied. Electron microscopy revealed that VMY22 has an icosahedral head (59.2 nm in length, 31.9 nm in width) and a tail (43.2 nm in length). Bacteriophage VMY22 was classifi ed as a Podoviridae with an approximate genome size of 18 to 20 kb. A one-step growth curve revealed that the latent and the burst periods were 70 and 70 min, respectively, with an average burst size of 78 bacteriophage particles per infected cell. The pH and thermal stability of bacteriophage VMY22 were also investigated. The maximum stability of the bacteriophage was observed to be at pH 8.0 and it was comparatively stable at pH 5.0-9.0. As VMY22 is a cold-active bacteriophage with low production temperature, its characterization and the relationship between MYB41-22 and Bacillus cereus bacteriophage deserve further study.
Received: 2 Feb 2015  Accepted: 30 Oct 2014  Published online: 1 Feb 2015
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