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Virologica Sinica, 30 (2) : 162-162, 2015
Erratum to: Binding of HIV-1 virions to α4β7 expressing cells and impact of antagonizing α4β7 on HIV-1 infection of primary CD4+ T cells
Erratum to: Virologica Sinica 2014, 29 (6): 381-392 DOI 10.1007/s12250-014-3525-8 Published online: 10 December 2014 In the original version of this article, the legend to Figure 3 was incorrect due to a mistake in typesetting. The Virologica Sinica staff apologizes for this error. The corrected legend is given below. VIROLOGICA SINICA 2015, 30 (2): 162 DOI 10.1007/s12250-015-3579-2 The online version of the original article can be found at http://dx.doi.org/10.1007/s12250-014-3525-8. Published online: 10 March 2015 Figure 3. Impact of blocking α4β7 by antibodies or down-regulating α4β7 by RNA interference on HIV-1 infection of CD4+ T cells. A: α 4β7 expression on CD4+ T cells cultured w/ or w/o RA treatment was measured by FCM. B: Virus infection of RA-treated CD4+ T cells in the presence or absence of antibodies. After pre-incubation with anti-α 4 HP2/1, anti-α4β7 Act-1, anti-CD4 RPA-T4 or control IgG (each 5 μg/mL) for 1 hour at 37 oC, 2 × 105 CD4+ T cells were infected with 2 ng p24 of HIV-1 BaL for 3 hours, followed by extensive washes to remove unbound virus. Antibodies were always present as indicated through the infection and subsequent culture procedure. Culture supernatants were collected every three days p.i. until 12 days p.i., lysed and stored at -80 oC until p24 measurement. C-F: α 4β7-activated CD4+ T cells were transduced with lentivirus carrying shRNA targeting integrin α 4 or HIV-1 coreceptor CCR5, non-targeting shRNA (scrambled) or vector alone. 4-6 days post transduction, the expression of α4β7 (D) and CCR5 (E) were analyzed on positively transduced GFP+ lymphocytes (C). F: The positively transduced lymphocytes were sorted and subsequently infected with HIV-1 BaL. Virus infection and sample collection was conducted as described in (B). G: Infection of HIV-1 BaL in CD4+ T cells following transduction with shRNAs targeting β1 or a non-targeting scrambled shRNA. The efficiency of β1-specific shRNA to down-regulating β1 expression on CD4+ T cells was shown by the inset. Data shown are representative of three independent experiments using CD4+ T cells derived from different donors. For B and F, each condition was performed in sextuplicate and expressed as mean ± SD. *p < 0.05 **p < 0.01 and ***p < 0.001, compared to virus infection of CD4+ T cells in the presence of control IgG (B) or CD4+ T cells transduced with scrambled shRNA (F, G). ns, not significant.
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Received: 10 Mar 2015  Accepted: 10 Mar 2015  Published online: 10 Mar 2015
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