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Virologica Sinica, 30 (3) : 231-233, 2015
Characterization of an infectious pancreatic necrosis virus from rainbow trout fry (Onhorhynchus mykiss) in West Ukraine
1. Laboratory of Biotechnology in Aquaculture, Institute of Fisheries
of the National Academy of Agrarian Sciences of Ukraine,
Kyiv 03164, Ukraine
2. Department of Molecular Biology, Biotechnology and Biophysics,
Institute of High Technologies, Taras Shevchenko National
University of Kyiv, Kyiv 01601, Ukraine
3. Laboratory of Physico-Chemical Biology, ESC Institute of Biology,
Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine
 Correspondence: rud_yuriy@ifr.com.ua
In this report, an aquatic birnavirus was isolated from rainbow trout fry, Onhorhynchus mykiss, during a fish health inspection in fish-farms in the west region of Ukraine near the rivers Siret and Cheremosh(Chernivtsi region). Preliminary examination of infected fish revealed a range of lesions, particularly in pancreatic tissue. Morphological changes, such as vacuole enlargement and cell rounding, were caused by viruses in appropriate cell lines. Investigation by electron microscopy demonstrated that the isolated virus was ultrastructurally similar to infectious pancreatic necrosis virus(IPNV). In addition, the nucleotide sequences of the 1120 bp VP2 gene fragments were analyzed and the identity of the isolated IPNV to strain Sp was revealed. The identity of nucleotide sequences was 97%–99%; the isolates of Sp strains most closely related to the Ukrainian IPNV isolate were found in Norway.
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  Published online: 29 Jun 2015
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