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Author [FANG Qi] article list of Virologica Sinica
Currently, three predominant subtypes of influenza virus are prevalent in pig populations worldwide: H1N1, H3N2, and H1N2. European avian-like H1N1 viruses, which were initially detected in European pig populations in 1979, have been circulating in pigs in eastern China since 2007. In this study ..., Abstract
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Viral nonstructural proteins in both enveloped and non-enveloped viruses play important roles in viral replication. Protein NS38 of Grass carp reovirus (GCRV), has been deduced to be a non-structural protein, and, consistent with other reoviruses, is considered to cooperate with the NS80 protein in ..., Abstract
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By Using the modified method to extract Rice dwarf virus virions from infected rice leaves, a concentration 3.854mg/mL of purified virus virions reached, and the Rabbits was immunized with purified virions, yielding high titer antiserum; 1:20480 by the indirect enzyme-linked immunosorbent assay ..., Abstract
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Five Tobacco mosaic virus isolates, obtained from tobacco leaves showing typical symptom in Qujing, Honghe, Dali, Chuxiong and Yuxi in Yunnan province, were selected and studied from 637 TMV samples. Using a pair of primers specific for TMV-U1 strain, a specific fragment of 530bp including the TMV ..., Abstract
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An icosahedral virus was found in the ultra thin section of spleen, kidney, liver, gill of the outbreak infectious Siniperca chuatsi by electron microscope. The virions of section show hexagon, about 125 150 nm in diameter, and have envelope outside. Cell culture indicated that CIK cell was ..., Abstract
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The morphogenesis of the Grass Carp Reovirus (GCRV) was studied in CIK cells. The CIK cells were infected by the GCRV at MOI (multiplicity of infection) of 5 10 PFU/Cell. It was demonstrated that the subviral particles without outer layer proteins could be seen in the cytoplasm at 4 hours post ..., Abstract
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Grass Carp Hemorrhage Virus (GCHV) was purified from infected fish cell culture by using different centrifugation. Its total genome was isolated by proteinase K treatment following phenol/chloroform extraction. The individual segment of GCHV dsRNA was separated by running 1% agarose gel ..., Abstract
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By studying the flue structure of GCHV (Grass Carp Hemorrhage Virus), It was found that the capsid of purified GCHV can be degraded spontaneously into its morphological units by prologing in a buffer of low ionic strength, but not very completely. The virus preparation was obtained from GCHV ..., Abstract
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The Grass Carp ( Ctenopharyngodon idellus) Hemorrhage Virus (GCHV) is able to cause slight haemagglutination of human type O blood cells. Serological tests showed that the GCHV is not antigeneally related to Reovirus and Rotavirus by cross reaction with ELISA,, Abstract
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A method for plaquing Grass Carp Hemorrhage Virus-873 has been studied. We tested inorganic(NaHCO_3) and organic(Tris, HEPES) buffer systems in open dish culture and found the combination of organic buffers with bicarbonate to be very promising for both cell growth and viral replication The CIK ..., Abstract
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