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Author [HONG Mei-Xian] article list of Virologica Sinica
Hemorrhagic fever with renal syndrome(HFRS)viral RNA in specimens of urine cells, serum and peripherial blood lymphoeytes(PBL)from patients was detected by dot-blot hybridization with photobiotin-labelled Rs cDNA probe. Positive results were obtained with 52 of 81 urine, 24 of 48 serum and 30 of 42 ..., Abstract
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After being infected with Japanese encephalitis virus (JEV), the rhesus monkeys appeareda series of symptoms and signs, and finally died of exhaustion of respiration and circulation. Two days after the temperature rised upto 39℃, the. monoclonal antibodies (McAb) were injected for therapy. The ..., Abstract
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A rapid IgM antibody capture (RMAC) ELISA using enzyme labeled one ofthe species specific McAb 2H_4 as tracer was studied for the early diagnosis.The antibody thus obtained was confirmed specific, because it was destructableby 2-ME, could be blocked by JEV antigen, and had no cross reactivity with ..., Abstract
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A method has been developed to purify HFRS virus structural protein. HFRS virus was first purified roughly by protamine sulfate and polyethylene glycol precipitation from brain tissue of suckling mice infected with HFRS virus strain 76-118 or strain Chen, and the roughly purified virus was then ..., Abstract
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We detected antibodies to HFRSV in 156 sera of 82 patients with HFRS diagnosed clinically by RPHI and IFA-IgG. 66 out of 82 cases showed positive, and 83.6% and 73.8% of them were 4 times higher than those of acute stage in antibody titer of convalescent serum by RPHI and IFA-IgG, respectively. ..., Abstract
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A 30,000-dalton structural protein(VP30)on herpes simplex virus type-2 (HSV-2) -infected BHK cell membranes was purified with an immunoadsorbent column consisting of the HSV-2 type-specific monoclonal antibody(McAb) CH-A9 coupled to Sepharose 4B. The Evidence shows that the VP30 has some properties ..., Abstract
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A hybridoma cell line(Mad-2) was produced by fusing mouse myloma cell line Sp2/O with the spleen cells immunized by HSV-1 SM44 strain infected BHK-21 cells and membrane protein antigen extracted from the HSV-1 infected ceils, the positive clone Mad-2 secreating monoclonal antibody(McAb) against ..., Abstract
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2-1 day old suckling mice were inoculated ic with 8 HFRSV strains (Chen, A_(216), A_9, H_(8205), C_4, R_(22), 76/118) respectively. The brain tissue of the mice was harvested immediately before death and prepared into HAN by sucrose-acetone method. The HA activity and HI reactivity were tested. It ..., Abstract
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A double sandwich ELISA has been developed to detect herpes simplex virus(HSV) antigen in 70 clinical specimens, A valuation on availability of Monoclonal Anti body (McAb) in rapidly detecting HSV antigen was set, The sensitivity of McAb ELISA was 20-30 TCID50/0.1ml. The concordance of detecting ..., Abstract
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