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Author [JI Xiao-Hui] article list of Virologica Sinica
The growth characteristics of Human herpesvirus 7 local strain YY5 were investigated in cord blood mononuclear cells (CBMCs) and CD4 + lymphoblastic cell line SUPT1. Virus growth was demonstrated by cytopathic effect (CPE), percentage of dead cells count, indirect immunofluorescence test with viral ..., Abstract
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The production of tumor necrosis factor alpha(TNF-a)induced by human herpesvims
7(HHV一7)G1asgow strain and Nanjing loca1 strain YY5 and HHV一6 GS strain in umbilica1 cord
and pheripheraI blood mononuclear cells cultures was studied by means of biologicaI activity assay.
It ..., Abstract
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The local strain of human herpesvirus 6 (HHV 6),named CN5, was used to study some immunological properties of infected cells.By the methods of indirect immunofluorescence test, APAAP and MTT, the morphological and kinetic character of viral antigen expression, the changes of CD antigens expressions ..., Abstract
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The effects of HHV - 6 infection in vitro on cytokines such as IL - 6, IL - 8 synthesis and NK activity of human peripherial blood mononuclear cels (PBMC) were studied by comparing CN8 strain with GS strain. Interleukin - 8 (IL - 8) could be detected as eary as 24 h, plateaued at 48h or so by ..., Abstract
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here are high levels of TNF-α in the supernatants of lymphocyte culture infected with HHV-6 GS strain and the Nanjing loeal strains.TNF-α was detected by two methods:biological ac-tivity assay using L929 cells and ELISA.HHV-6 can induce TNF-α with in 24 h,maximal re-lease of TNF-α occurred ..., Abstract
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In vitro hunian monocyte-line U937 cells and mouse peritoneal macrophages were inoculatedwith HSV-1,which were persistently treated with difterent cytokines since several days beforeor after inoculation. The effect of these cytokines on the infection of monocyte-niacrophages withHSV -1 was studied ..., Abstract
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eripheral blood samples from patients with exanthem subitum and lymphoproliferative dis-orders were collected.Mononuclear cells were separated from patients blood and were cultured invitro.As a result,virus strains were isolated from the peripheral blood mononuclaear cells of sevenpatients with ..., Abstract
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Cells of human monocyte-line U_(937)and human T-lymphocyte-line HSB_2 inoculated withHSV-1 were studied by observing the dynamics of virus titer,cellular proliferation and cy-topathogenic effect,expression of virus antigen and existing time of virus DNA. The resultsshowed that after ..., Abstract
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PCR was adapted to detect HSV-DNA sequence in samples from 72 petients with clironic cervici-tis and 30 healthy cotrols,and compared with McAb-ELISE and virus isolation(VI).The plsitive rateof HSVby these three methods was 28.4% 22.5% 20.6% respotively,and coinciuent rates a-mong threelmired ..., Abstract
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