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Author [LIAO Qing-jiao] article list of Virologica Sinica
A recombinant HCV RNA-dependent RNA polymerase (RdRp, NS5B protein) was expressed in E. coli strain BL21(DE3). The purified protein was used to investigate the RNA syntheses from the 3′ terminal sequences of HCV positive and negative strand RNA in vitro. The positive strand RNA did not direct the ..., Abstract
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Coding sequences of Transfusion transmiffed virus(TT virus or TTV) ORF2 was amplified by PCR, using plasmid pET-His-TTV2 containing the full-length ORF2 of TTV as template. The amplified fragment of 606bp was cloned into plasmid pEGFP-N1 and GFP-VP2 fusion protein was expressed. The recombinant ..., Abstract
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Full-length NS5A gene of Hepatitis C virus(HCV) was amplified by PCR, using the plasmid pBRTM/HCV 1-3011 containing HCV full-length open reading frame(ORF)as template, and cloned into the eukaryotic expressing plasmid pcDNA3.1(-)by DNA recombination technique. The recombin- ant vector was ..., Abstract
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Recombinant plasmid pCNS2 was constructed by inserting Hepatitis C Virus full-length NS2 gene between Xho I and Hind III sites of an eukaryotic expression vector pCDNA3.1(-). The recombin- ant plasmid pCNS2 was transfected to Huh-7 cells with LipoVecTM and HCV NS2 mRNA and protein produced in ..., Abstract
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Abstract:Recombinant plasmid pCDNA3一Core was constructed by inserting Hepatitis C virus(HCV一1 1
core gene into the site between EcoR I and Xba I of eukaryotic expression vector pCDNA3.The
recombinan t plasmid pCDNA3一Core was tran sfected to COS一7 cellline by LipoVec .Expression of ..., Abstract
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