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Author [MI Ke-Yong] article list of Virologica Sinica
ovine viral diarrhea virus(BVDV)InL genomic RNA was used as a template to synthesizeDNA by reverse transcriptase.The ds cDNA was synthesized by RNase H-DNA polymerase,and waslsetrted into the Pst I site of the pUC8 vector by dC-dG homopolymeric tailing and ligation.After beig transformed to E.coli ..., Abstract
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The BVDV(Bovine viral diarrhea virus)RNA is ssRNA.After the virus was purified, the BVDV RNA was extracted hy phenol-chloroform-isoamyl aleohl. The DNA contamination in the extracted BVDV RNA was degraded by DNase l. BVDV RNA was further purified by oligo dTcellulose affinity chromotography and ..., Abstract
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A strain of bovine viral diarrhea virus (BVDV) was isolated from epidemic area of lamb in Inner Mongol in recent years During 1983-1985, we have deteted 229 shares of the fecal samples suffering from this illness in Inner Mongol, the shares of the viral samples account for 59 pereent.These virions ..., Abstract
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The ~(32)P-cDNA Probes of Citrus Exocortis Viroid (CEV) and Chrysanthemum Stunt Viroid (CSV)RNA has been synthesied respectively and usedfor detecting some samples that may contain relative viroids. This is a sensitive and rapid method for diagnosing early viroid disease as compared with biology ..., Abstract
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