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Author [WANG You-Chun] article list of Virologica Sinica
The serum samples and corresponding cervical swabs were collected from 50 women with genital warts from Tianjin city, China. The neutralizing antibodies against HPV-16, -18, -58, -45, -6 and -11 in serum samples were tested by using pseudovirus-based neutralization assays and HPV DNAs in cervical ..., Abstract
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To develop national reference of HIV-1 P24 antigen and establish the corresponding standard, specimens collected from normal population, HIV cultivated cell lysates and patients infected with HIV or suspected infection, were detected for HIV antibody, HIV P24 antigen, HCV antibody and HBsAg. 21 ..., Abstract
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The objective of this study was to determine the relationship between the nucleic acid and antibody of HIV/HCV in a high-risk population. Plasma samples (320) were collected from drug users in Xinjiang and the nucleic acid and antibody of HIV/HCV were detected. A total of 38% of the subjects were ..., Abstract
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PCR method was used to Construct multi-epitope gene ss1c containing HBV S(1-222AA),preS1(10-50AA)and C(1-30AA) gene.The fusion ss1c gene was cloned into the expression vector pPIC3.5k.The recombinant vector was transformed into the host cell of GS115 with electroporation method.After screening with ..., Abstract
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To investigate HIV genotypes isolated from blood donors in Beijing, the samples positive for anti-HIV antibody with ELISA were confirmed by HIV blot. All 26 samples were confirmed positive for anti-HIV antibody. RNA from those samples were detected with RT-PCR. 20 of them were positive for HIV RNA. ..., Abstract
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Partial genomes of three strains of sporadic hepatitis E virus (Ch T11,Ch T21,Ch T40) were cloned and sequenced. The nucleotide and putative amino acid homology of Ch T11,Ch T21,Ch T40 and other HEV strains were 78.3 81.3% and 95.8 97.9%, respectively. The nucleotide homology between Ch T21 and Ch ..., Abstract
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Thirteen indeterminate HBsAg samples were tested with 21 sorts of HBsAg kits, anti HBs and anti HBc kits. HBV DNA was detected with nested PCR. The results showed that all of HBsAg kits can not easily detected HBsAg from some HBV DNA positive samples. It indicated that HBsAg concentration may be ..., Abstract
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Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of "a" determinantof ..., Abstract
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