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Author [XIE Lian-Hui] article list of Virologica Sinica
The full-length cDNA of the genome segment(S9) of Rice gall dwarf virus Xinyi isolate(RGDV-C) was cloned and the complete nucleotide sequence was determined.The results showed that S9 had 1202 nucleotides(AY556483) encoding a polypeptide of 323 amino acids with an Mr of 35.6kDa.The S9 had the same ..., Abstract
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It is very important to understand the laws of Tobacco mosaic virus(TMV) inactivation under different water bodies and temperatures. Choosing the typical plant virus TMV as a model virus, the inactivation dynamics of it in Min River water, tap water, super-pure water, life sewage and sterile sewage ..., Abstract
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The efficiency of adsorption and removing Poliovirus 1(PV1) and bacteroides fragilis phage(B.fp) by nature and modified sand in the conditions of different environment pH, various mental salts and concentration variation, exist of humic acid in tap and distilled water were studied in this paper. ..., Abstract
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Rice grassy stunt virus sp gene was transformed into rice by Agrobacterium mediated transformation. PCR analysis and Southern blot hybridization by using the genomic DNA of regenerated plants showed that the sp gene was transformed into rice. By using RT-PCR Southern blot hybridization, it was ..., Abstract
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It is very important to optimize the operating conditions of virus concentration from water body for evaluating virus’s pollution and removing efficiency. In this paper, an optimizing method of virus concentration from water body was established. It was to adopt the way of throwing clay of ..., Abstract
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A antiviral protein y3, from mushroom Coprinus comatus, could inactivate Tobacco mosaic virus (TMV) in vitro. The inhibition rate against is 50.0% when the concentration of y3 is 2.0μg/mL. When RNase was added to the mixture of TMV and y3, the infection rate of TMV was 61.74%, decreased by 38.26% ..., Abstract
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Abstract:The complete nucleotide sequences of the RNA2 of two Chinese isolates of Rice stripe virus
are determined.One is isolated from endemic sites at Chuxiong(cx),Yunnan Province。the other is
isolated from outbreak sites at Hongze(HZ),Jiangsu Province.Th e total nucleotide ..., Abstract
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Methods of ELISA, nonradioactive molecular hybridization and RT PCR were applied in the detection of rice grassy stunt virus (RGSV). The detection sensitivity of indirect ELISA using antiserum against fusion protein GST NC was 1 mg of infected leaves or 84 ng of purified virus. The method of dot ..., Abstract
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A high virulent isolate of White Spot Disease Virus has been got in Fuzhou. The virion is in cytoplasm. Intact virion is enveloped and bullet shaped, d=80-100 nm. There is no inclusion body in cell, but sometime a particular structure named occlusion body (some virions are occluded by membrane) in ..., Abstract
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Based on the known RNA sequence of rice grassy stunt virus , IRRI isolate (RGSV IR), the cDNA of nucleocapsid protein (NCP) gene was obtained by RT PCR, with genomic RNAs of Shaxian isolate (RGSV SX) as template. The cDNA was then cloned into pGEM T vector and sequenced. The results showed that the ..., Abstract
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The RNA4 intergenic regions of two isolates of rice stripe virus (RSV) in China (de signated as RSV JN and RSV YL) were amplified by RT PCR and cloned into pGEM T easy vector. Sequence analysis showed that they were all 654 bp in length, and shared only 92% identity at the nucleotide level. ..., Abstract
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人和动物病毒污染水体的研究已有多年 ,并取得了可喜的成果 ,其研究已从单纯的环境监测和卫生评价向机制和规律等纵深方向发展。分子生物学方法已深入到水病毒学领域 ,利用基因探针和扩增技术检测水体病毒已成为重要的研究方法 。然而针对水体植物病毒污染及其环境效应..., Abstract
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An isolate of rice stripe virus (designated as RSVYL) was purified. The particlesshowed to be pleomorphisms under electron microscope, mainly branched filamnts of about 80-250 nm in length and abeut 8 nm in width. There are also some open circular filaments of 3nm and 8 nm in width, and some ..., Abstract
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According to the differential pH precipitation of proteins, two methods were established for purification of disease specific protein (SP) in rice plants infected by rice stripe virus (RSV), a member of \%Tenuivirus.\% The yields obtained by the two methods were 0.8 and 2.0 mg SP per g of infected ..., Abstract
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Comparative studies on biological properties, physical and biochemical properties, morphology and serological relationships of five cucumber mosaic virus (CMV) isolates (PE,PE2,PEf,PC,PF) infecting Passiflora edulis, P. edulis × P.edulis var. flavicarpa, P.edulis var. flavicarpa, P.caerulea ..., Abstract
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he plant expression vector pROK II containing antisense ribozyme gene of rice dwarf virus(RDV)S5 was transferred to rice immature embryo by microprojectile bombardrnent.The resis-tant calli were obtained in the presence of G418. Two or three months later these calli could differ-entiate to shoot ..., Abstract
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Narcissus mosaic virus(NMV) was obtained from the mixed infected narcissus plants and purified from the inoculated leaves of Chenopodium amarnticolor. The procedures of purification involved precipitation with 6% PEG ( M. W. 6000 ), differential centrifugation and 20—50% sucrose density-gradient ..., Abstract
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