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Author [XIE Tian-En] article list of Virologica Sinica
The development of Dendrolimus punctatus wenshanensis Cytoplasm Polyhedrosis Virus (DpwCPV) insecticide is reported. The formulation is an oil in water emulsion and the concentration of polyhedrosis is 2.5×10 9 PIB/mL. The auxiliary materials come from the agriculture products. It is safety ..., Abstract
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The part of effects and characters of infection with Buzura suppressaria nuclear polyhedrosis virus(BsNPV)for Bs484 cells in the different media and cultural time were reported in this peper.The re sults showed that the types of media and the time after passage for Bs484 cells could produce ..., Abstract
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Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by ..., Abstract
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The paper reports paroceneria furva single nuclear polyhedrosis virus(PfNPV)that was not described before.The size of mlyhe0m inclusion bodies(Pros)raiages from 1.0um to 1.35um.The virions are rod—shaped,approximately 371× 73nm., Abstract
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By the polyacrylamide gel electrophoresis(PAGE)and thin-layer scanning,the esterases of thefourth instar larvae of the Buzura suppressaria and Bs484 cells were investigated after infection with nuclear pelyhedrosis viruses,The amount of the zymograms for mid-gut esterases in diseased larvae ..., Abstract
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The oil tree moth (B. suppressaria larvae) was grown to 4 instar using a seml-synthetic artificial diet. then infected with (BsNPV) inoculum propagated in the insect cell cultures. 7 days p.i., the virus-infected larvae were collected and virus (PIB) was purified by differential centrifugation. ..., Abstract
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This thesis reported the following infective charaeters of Buzura suppressatia nuclear polyhedrosis virus (BsNPV) in Bs484 cell line: 1. The viral infection rate was the highest when the virus was inoculated in Bs484 cells had been subeultured for 3—4 days. 2. The viral inocuiating quantities ..., Abstract
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The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA ..., Abstract
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The polyhedrin gene of tung-oil geometrid, Buzura suppressaria,nuclear polyhedrosis virus(BsNPV) was localized on BamHI-B, BglI-A, Bgl Ⅱ-F, EcoRI-P, HindⅢ-A, KpnI-I, PstI-D, XbaI-A or B and XhoI-F/G fra-gments under low stringence(40% formamide, 35℃), using the recombinantplasmid of AcNPV DNA ..., Abstract
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A Cytoplasmic polyhedrosis virus (CPV) from the Dendrolimus punctatusWenshanensis was studied on some characterizations of fine structure and bio-chemistry. Most of polyhedra were hexhedral, less quadrilaterly and appro-ximaterly spherical. The size of polyhedra showed a significant ..., Abstract
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We isolated a new baculovirus—Pavoenerin orient Nuclear Polyhedrosis Virus (PoNPV). The polyhedral IBs range in size from 1.06—2.42μm. The virions are rod-shoped, approximately 385×55μm. The rate of death was 95%when P.orient laraves were infected with PoNPV., Abstract
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The plaque assay was carried out for Buzura suppressaria nuclear polyhedrosis virus (BsNPV) using cell line Bs-484. Results showed that the sensitivity of this plaque assay method was similar to that of TCID_(50) method., Abstract
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Aspartyl-Prolyl bonds in proteins could be selectively hydrolyzed with 75% formic aeid. The in situ aeidie cleavage procedure based on acid lability of Asp-Pro bonds is very important in sequence analysis of proteins and comparative studies of related or similar proteins, Limited cleavage of ..., Abstract
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There have not been formally reports of Bombyx mori densonucleosis virus infccting insect cell lines. In the present paper three insect overy cells of B.suppressaria, S. frugiperda and M.brassicae infected with Bombyx mori densonucleosis virus.The result is that cytopathic effect was only observed ..., Abstract
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Buzura suppressria NPV has been the most widely used virus by artificial dissemination of NPV in the tea plantation with trial conducted in Hubei, Guangdong. Jiangxi go on. In puqi, in 1979, 1981, 1983. dosages of 2×10~6PIB/ml, were tested and Tea garden were sprayed to 750 liter/ha. At 10 days ..., Abstract
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This experiment is basad on the infection of Buzura Suppressaria ovary cell line by Buzura Suppressaria NPV. After static incubation of the infected cell line at different temperatures. 0℃, 15℃, 20℃, 26℃, 28℃,30℃, and 37℃, the cytopathologieal effect have been observed by the determination ..., Abstract
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The assembly processes of the philosamia cynthia ricini D. NPV are observed by electron microscopy of sections of infected cell cultures. The synthesis and envelopment of the virus nucleoeapsid and stage in the formation of the polyhedral inclusion bodies are described, Studies of the Philosamia ..., Abstract
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