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Author [
Xiang-tao LIU
] article list of
Virologica Sinica
Indirect ELISA with Recombinant GP5 for Detecting Antibodies to Porcine Reproductive and Respiratory Syndrome Virus
Yan Chen
,
Hong Tian
,
Jian-Hui He
,
Jin-Yin Wu
,
You-jun Shang
,
Xiang-tao Liu
Porcine reproductive and respiratory syndrome is caused by the PRRS virus (PRRSV), which has six structural proteins (GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay (ELISA) and other methods. Toward this goal, we ...,
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2011 Vol. 26 (1)
: 61-66
Genetic Variation Analyses of nsp2 Gene of PRRSV in Ningxia Hui Autonomous Region of China
Hong TIAN
,
Jing-yan WU
,
Shuang-hui YIN
,
You-jun SHANG
,
Zi-ping MAN
,
Na ZHAO
,
Ye JIN
,
Xiang-tao LIU
To gain a better understanding of the genetic diversity and evolution of PRRSV in the Ningxia Hui Nationality Autonomous Region (Ningxia) of China, the nsp2 genes from a series of PRRSV strains collected from the region in 2007 were partially sequenced. These sequences were then analyzed along with ...,
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Springerlink
2009 Vol. 24 (3)
: 221-226
Amplification and Characterization of Bull Semen Infected Naturally with Foot-and-mouth Disease Virus Type Asia1 by RT-PCR
Jun-jun SHAO
,
Hui-yun CHANG
,
Tong LIN
,
Guo-zheng CONG
,
Jun-zheng DU
,
Jian-hong GUO
,
Hui-fang BAO
,
You-jun SHANG
,
Ya-min YANG
,
Xiang-tao LIU
,
Zai-xin LIU
,
Ji-xing LIU
Abstract: To investigate the security of semen biologically, 15 bull semen samples were collected (of which 5 exhibited clinical signs of Foot-and-mouth disease) and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected bulls were contaminated by ...,
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2008 Vol. 23 (5)
: 378-382
Expression of Major Antigen Domains of E2 Gene of CSFV and Analysis of its Immunological Activity*
Hong TIAN
,
Xiang-tao LIU
,
Jing-yan WU
,
You-jun SHANG
,
Tao JIANG
,
Hai-xue ZHENG
,
Qing-ge XIE
E2 is an envelope glycoprotein of Classical swine fever virus (CSFV) and contains sequential neutralizing epitopes to induce virus-neutralizing antibodies and mount protective immunity in the natural host. In this study, four antigen domains (ABCD) of the E2 gene was cloned from CSFV Shimen strain ...,
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Springerlink
2008 Vol. 23 (4)
: 247-254
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