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Author [YE Lin-bai] article list of Virologica Sinica
HBV X gene was amplified by PCR and cloned into the prokaryotic expressing vector pET-his and the eukaryotic expressing vector pcDNA3.1(-). E.coli BL21 (DE3) were transformed by the recombinant plasmid pET-his-HBx.. HBx protein was expressed by IPTG induction and formed insoluble inclusion bodies ..., Abstract
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A recombinant HCV RNA-dependent RNA polymerase (RdRp, NS5B protein) was expressed in E. coli strain BL21(DE3). The purified protein was used to investigate the RNA syntheses from the 3′ terminal sequences of HCV positive and negative strand RNA in vitro. The positive strand RNA did not direct the ..., Abstract
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Coding sequences of Transfusion transmiffed virus(TT virus or TTV) ORF2 was amplified by PCR, using plasmid pET-His-TTV2 containing the full-length ORF2 of TTV as template. The amplified fragment of 606bp was cloned into plasmid pEGFP-N1 and GFP-VP2 fusion protein was expressed. The recombinant ..., Abstract
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To establish a technique for preparing rabies vaccine by perfusion culture on microcarriers in bioreactor, a 5 litre bioreactor was filled with 199 cell culture medium which contains 10g/L of microcarriers. Then Vero cells were inoculated with a final cell density of 1×105/mL . After 7 days of ..., Abstract
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Full-length NS5A gene of Hepatitis C virus(HCV) was amplified by PCR, using the plasmid pBRTM/HCV 1-3011 containing HCV full-length open reading frame(ORF)as template, and cloned into the eukaryotic expressing plasmid pcDNA3.1(-)by DNA recombination technique. The recombin- ant vector was ..., Abstract
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Recombinant plasmid pCNS2 was constructed by inserting Hepatitis C Virus full-length NS2 gene between Xho I and Hind III sites of an eukaryotic expression vector pCDNA3.1(-). The recombin- ant plasmid pCNS2 was transfected to Huh-7 cells with LipoVecTM and HCV NS2 mRNA and protein produced in ..., Abstract
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To establish an effective, economical and simple technology for preparing rabies viral antigen that can be used to produce horse anti-rabies serum, rabies virus strain was inoculated in vero cells cultured with medium containing 10% horse serum and was propagated with 199 culture medium containing ..., Abstract
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The E2 gene of Hepatitis C Virus was amplified by PCR.and cloned into the pQE30 at the
downstream of LacZ promoter.The recombinan t vector DNA was introduced into the E.coli盯 109 cell
to create the JM109 pQE2.The HCV E2 protein fused to 6 His peptides with molecular weight of
35kDa ..., Abstract
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A rapid method, micronucleus assay which proposed in the scoring of chromosomal aberrations has been dealt with the safety evaluation of insect viruses of tea pests(Biston marginata NPV, Euproctis pseudoconspersa NPV and Buzura suppressaria NPV)in mice. By scoring the frequency of micronuclei in ..., Abstract
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Six kinds of baculoviruses(BsNPV1, BsNPV2, BtNPV, EpNPV, PrGV, PxGV) are purified by centrifugation using sucrose gradient,Roughly purified granulins and polyhedrins are obtained by having the inclusion body solubilized in dilute alkaline salne after possible alkaline protease activity is ..., Abstract
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