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Author [ZENG Ying-chun] article list of Virologica Sinica
HBV X gene was amplified by PCR and cloned into the prokaryotic expressing vector pET-his and the eukaryotic expressing vector pcDNA3.1(-). E.coli BL21 (DE3) were transformed by the recombinant plasmid pET-his-HBx.. HBx protein was expressed by IPTG induction and formed insoluble inclusion bodies ..., Abstract
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A recombinant HCV RNA-dependent RNA polymerase (RdRp, NS5B protein) was expressed in E. coli strain BL21(DE3). The purified protein was used to investigate the RNA syntheses from the 3′ terminal sequences of HCV positive and negative strand RNA in vitro. The positive strand RNA did not direct the ..., Abstract
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To establish an effective, economical and simple technology for preparing rabies viral antigen that can be used to produce horse anti-rabies serum, rabies virus strain was inoculated in vero cells cultured with medium containing 10% horse serum and was propagated with 199 culture medium containing ..., Abstract
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