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Author [ZHANG He-Ling] article list of Virologica Sinica
The DNA of Canine Parvovirus Inner Mongolia isolate (CPV IM) was isolated from the enteric lysate of virus infected dog showing enteritis symptoms. The VP2 gene of CPV IM was amplified by PCR using designed and synthesized two primers and cloned into plasmid pUC19 at Bam H I/ Sac I sites. ..., Abstract
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According to the genomic sequence of PLRV S, two pairs of specific primers were designed and synthesized. The cDNA of the replicase gene of PLRV Chinese isolate (PLRV Ch) was synthesized and amplified by RT PCR using the viral RNA as a template. The synthesized 3′ and 5′ cDNA fragments ..., Abstract
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A hammerhead structure ribozyme was designed and synthesized to potato leafroll virus Chinese isolate (PLRV - Ch) genome within 356 to 358 nt "GUC" in coat protein (CP) gene. DNA sequence encoding the ribozyme was inserted into the position downstream from SP6 promoter of in vitro transcription ..., Abstract
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Two specific primers were designed and synthesized according to the genomic sequence ofPLRV reported in literature,The first strand of cDNA was synthesized by reverse-transcriptionusing RNA of PLRV Chinese isolate(PLRV-Ch)as a template,followed by PCR amplification.The synthesized cDNA was cloned ..., Abstract
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The cDNA of potato leafroll virus coat protein gene and cDNA by random primer were used as probe labeled with 32p by nick translation., Abstract
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weet potato feathery mottle virus(SPFMV)was inoculated to I.selosa to propagare by graftingand was purified wirh 0.2mol/L pH7.2 PBK.Further purification of SPFMV was conducted by sucrose cushion and sucrose density gradient centrifugation.Purified SPFMV had a OD260/280 ratio of1.25.Rilbbit was ..., Abstract
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Purified potato virus Y, X, and S were detected by indirect enzyme-linked immunosorbent assay (ELISA) on nitrocellulose membranes. Potato virus Y, X, and S in infected tobacco, sprouts of potato tubers, rose end of dormant tubers were detected respectively. The results indicated that the small ..., Abstract
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A coat protein gene eDNA of a PLRV isolate infected commercial potato cultivar "Purple Flower White" was synthesized by reverse transcription followed by Polymerase Chain Reaction amplification with synthesized 20merand 30mer primers. The synthesized eDNA was cloned in plasmid pUC19 in JM103. The ..., Abstract
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The hybridoma cell lines secreting monoclonal antibodies to Potato Leafroll Virus (PLRV) were established by fusion of myeloma cells SP2/0 with spleen cells of BALB/C mouse immunized with purified PLRV by direct spleen injection followed by two tail injections. The hybridoma were screened ..., Abstract
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The microslide gel double diffusion test was used to detect potato leaf-rollvirus (PLRV). It was found to be sufficiently sensitive for the detection ofPLRV in extracts of infected plants. A 1: 4 dilution of extract of PLRV infectedpotato stem could be detected unambiguously. The sensitivity of ..., Abstract
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