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Author [ZHANG You-Qing] article list of Virologica Sinica
A general cDNA library which was from infected larvae dueing the latent periods has been constructed. The first cDNA strand was synthesized from total RNA by M MLV transcriptase. After oligo(dG) tailing the cDNA was amplified by polymerase chain reaction. The double strands cDNA were ligated to ..., Abstract
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By the polyacrylamide gel electrophoresis (PAGE) and thin-layer scanning, the esterases (EST) of the third instar larvae of the Helicoverpa armigera were investigated in qualityand quantity after infection with nuclear polyhedrosis viruses (NPV). The changes in sort andcontent of hemolymph EST were ..., Abstract
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Total RNAs were isolated from Heliothis armigera larvae infected with Heliothis armigeraNuclear Polyhedrosis Virus(HaNPV) late after infection(60 h postinfection),800 μg total RNAswere obtained from 1g larvae by improved granidinium isothiocgarate method.The whole viralDNA was purified from HaNPV ..., Abstract
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The toxicity of a strain of Spodoptera tilura nuclear polyhedrosis virus(SINPV)was tested.The 4th instar larvae of Spodoptera titura were used to carryout the bioassays.LC5e=2.014×104PIBs/eachlarva.The genomic DNA was digested with three restriction endonucleases BamHI,EcoRI and PstI,and 17,22 and ..., Abstract
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Enzyme-linked Immunosorbent Assay(ELISA) has a higher sensitivity, speciality and simplicity, in particular, it is convenient in large-scale observation. The water and soil in the environment which produced and used Heliothis armigera NPV inssecticide were detected by this assay. The contents of ..., Abstract
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