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Articles list with the same subject [质型多角体病毒] of Virologica Sinica
As a major protein of cypovirus capsid shell,VP5 performs as the clamp protein to stabilize the capsid shell structure (Yu et al.,2008).And as a part of viral RNA (vRNA) replication machinery,VP5 has been found to possess an ATP-independent RNA chaperoning activity (Yang et al.,2014).Here,we report ..., Abstract
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The nucleotide sequence of segment 9 of Dendrolimus punctatus cypovirus (DpCPV) strain Hunan, which encodes the viral non-structural protein (NS5), was tagged with his-tag and expressed by pET32a (+). The recombinant protein was subsequently purified to homogeneity. By using the EMSA, which allows ..., Abstract
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To express the polyhedrin (the tenth segment, S10) of Dendrolimus punctatus Cypovirus (DpCPV) and investigate the localization of the polyhedrin in eukaryote, The polyhedrin gene was amplified from DpCPV by RT-PCR and cloned into pET-28a vector. The polyhedrin was then expressed ..., Abstract
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Abstract:DendrolimUS punctatus polyhedra were purified from infected Dendrolimus punctatus Larvae
using density centrifugation.Dendrolimus punctatus cytoplasmic polyhedrosis viruses(DpCPV)were
subsequently extracted from polyhedra by SDS treatment,followed by l% agrose gel ..., Abstract
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Abstract:Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)genome dsRNA wag
extracted an d S4 was separated.RT—PCR amplification technique Wag adopted to clone the viral
genomic dsRNA S4 flor sequence determination.The results showed:the full length of S4 is 3262bp,it ..., Abstract
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For the synthesis of cDNA of Dendrolimus punctaus CPV (DpCPV)NS5 protein,the primers
were designed on the basis of the RNA sequmce of BmCPV一1(I strain)segment 9.After the RT—PCR
the amplified cDNA was cloned into the pMD18一T. NS5 protein gene of DpCPV was found to be 963 ..., Abstract
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Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)was purified from infected
Dendrolimus punctattts and the genomic dsRNA segments were subsequently extracted. Segment 8
dsRNA was purified by low melting-temperat agraose.A single amino-linked modified oligonucleotide
(primer ..., Abstract
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CPVs belong to the genus Cypovirus in the family Reoviridae and infect midgut epithelial cells of the wide range of insects.For the synthesis of cDNA of Dendrolimus punctatus CPV (DpCPV) polyhedrin gene,the primers were constructed on the basis of the terminal RNA sequence of BmCPV segment 10,After ..., Abstract
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Argyrogramma agnata has been selected as a substitutive host insect for producing Den
drolimus punctatus Cytoplasmic Polyhedmsis Virus(DpCPV),In our experiment.it is very suscepti—
ble to DpCPV.The DpCPV produced in A.agnata is designated Aa-DpCPV The cytoplasmic poly—
hedra ..., Abstract
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The development of Dendrolimus punctatus wenshanensis Cytoplasm Polyhedrosis Virus (DpwCPV) insecticide is reported. The formulation is an oil in water emulsion and the concentration of polyhedrosis is 2.5×10 9 PIB/mL. The auxiliary materials come from the agriculture products. It is safety ..., Abstract
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The sixth stadium larvae of massonpine caterpillar in the overwintered,first and third generationswere infected with CPV of Dendrolimus spectabilis at the same ineeulation of 1×107 PIB/ml in forest.The results suggested that the larvae of overwintered generation could be chosen as the best carrier ..., Abstract
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The Lymantria,xylina Swinhoe cytoplasmic polyhedra xhows a quite difference ranging from 0.8 to2.lμm in diameter.The virions were randomly distributed in a polyhedron;The virions show icosahedron, and about 60 nm in diameter with l2 spikes.By the analysis of SDS-PAG E,the polyhedral pro-teins ..., Abstract
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A Cytoplasmic polyhedrosis virus (CPV) from the Dendrolimus punctatusWenshanensis was studied on some characterizations of fine structure and bio-chemistry. Most of polyhedra were hexhedral, less quadrilaterly and appro-ximaterly spherical. The size of polyhedra showed a significant ..., Abstract
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The larvae of Ostrinia furnacalis are susceptible to the infection with the nuclear polyhedrosis virus of Plusia agnata(PaNPV), the Antheraea pernyi NPV (ApNPV)and the cytoplasmic polyhedrosis virus of Dendrolimus spectabilis(DsCPV). The ID_50 for the 1st instar larvae infected with DsCPV is ..., Abstract
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The paper reports observations on a cytoplasmic polyhedrosis virus (CPV) from Bombyx mori infecting Bombyx mori cells (Bm-N)by electron microscopy. In the early stage of infection, the electron dense virogenic stroma containing virions formed in the cytoplasm of infected cells. Inclusion bodies ..., Abstract
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