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Articles list with the same subject [Detection] of Virologica Sinica
The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus (ZIKV) and in preventing serious neurological complications of ZIKV infection. In this study, we established micro-droplet digital polymerase chain reaction (ddPCR) and real-time ..., Abstract
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Bats carry a variety of viruses,and some of them cause public health problems.Macau,which is famous for its gambling industry,has a complex population structure.The globalization in such an international metropolis has enhanced the chance of disease transmission.Therefore,surveillance of zoonotic ..., Abstract
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A quantitative real time reverse-transcription polymerase chain reaction (qRT-PCR) assay with specific primers recommended by the World Health Organization (WHO) has been widely used successfully for detection and monitoring of the pandemic H1N1/2009 influenza A virus. In this study, we report the ..., Abstract
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To provide a foundation for the development of rapid and specific methods for the diagnosis of rabies virus infection, anti-rabies virus monoclonal antibodies were prepared and rabies virus nucleoprotein and human rabies virus vaccine strain (PV strain) were used as immunogens to immunize 6-8 week ..., Abstract
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A real-time monitoring reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the sensitive and specific detection of prototypic, prevalent North American porcine reproductive and respiratory syndrome virus (PRRSV) strains. As a higher sensitivity and ..., Abstract
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In this study, the loop-mediated isothermal amplification (LAMP) method was used to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). According to the PCV2 sequences published in GenBank, multiple LAMP primers were designed targeting conserved sequences of PCV2. ..., Abstract
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White spot syndrome virus (WSSV), Taura syndrome virus (TSV) and Infectious hypodermal and haematopoietic necrosis virus (IHHNV) are three shrimp viruses responsible for major pandemics affecting the shrimp farming industry. Shrimps samples were collected from 12 farms in Zhejiang province, China ..., Abstract
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Postweaning multisystemic wasting syndrome (PMWS) is an important swine disease that is closely associated with porcine circovirus type 2 (PCV2). The capsid protein (Cap protein) is a major structural protein that has at least three immunoreactive regions, and it can be a suitable candidate antigen ..., Abstract
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Three pairs of specific primers were designed to amplify the F2-1, F2-2 and XF2-2 truncated sequences of ORF2 which encodes the capsid protein of porcine circovirus type 2 (PCV-2). The F2-1 sequence had most of the NLS region of ORF2, but the F2-2 and XF2-2 genes had the NLS region deleted. ..., Abstract
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A simple and rapid assay for the detection of Classical swine fever virus (CSFV) was established using reverse transcription loop-mediated isothermal amplification (RT-LAMP). This study describes the amplification of the genomic RNA of CSFV under isothermal conditions (63℃) within one hour, using ..., Abstract
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Twenty five serotypes of Bluetongue virus (BTV) have been identified worldwide. Rapid and reliable methods of virus universal detection are essential for fighting against bluetongue (BT). We have therefore developed and evaluated a pair of primers which can detect various serotypes of BTV by ..., Abstract
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This paper describes the development and challenge of HIV/AIDS testing laboratory network and quality assurance system in China. At present, the HIV/AIDS testing laboratories includes three classes, the National AIDS Reference Laboratory, HIV/AIDS confirmatory laboratories and HIV/AIDS screening ..., Abstract
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DNA microarrays have been acknowledged to represent a promising approach for the detection of viral pathogens. However, the probes designed for current arrays could cover only part of the given viral variants, that could result in false-negative or ambiguous data. If all the variants are to be ..., Abstract
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A fluorescent quantitative PCR (FQ-PCR) method based on sequences of the conserved gE of the PRV genome was established and evaluated after selection of optimal reaction conditions. A pair of primers and a fluorescent TaqMan probe specific for gE gene were designed and used. We compared the FQ-PCR ..., Abstract
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In this study, an improved real-time RT-PCR was applied to quantification of Foot-and-mouth disease virus in vivo and in vitro. The results suggested that this method had a high sensitivity with up to less than 10 copies / reaction. Furthermore, this novel method was successfully used to quantify ..., Abstract
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Three pairs of primers P1/P2, P3/P4 and P5/P6 of porcine circovirus 2 were designed and synthesized to compare the sensitivity and specialization to amplify products of 404bp, 647bp and 1773 bp. Optimum dilution of PK15 cell genome infected with PCV 2 was achieved with 10-6ng, 10-5ng to yield from ..., Abstract
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Specific primers and probes were designed according to conservative and specific DNA sequence of CMV,LSV and LMoV.The probes were spotted on glass slides to form microarrays The Cy3-labled single strand DNA fragments prepared by dissymmetical RT-PCR were hybridized with the probes on the glass ..., Abstract
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Nucleic acid sequence-based amplification with electrochemiluminescent detection (NASBA/ECL) of avian influenza virus subtype H5 was developed,comparing with virus isolation in embryonated chicken eggs. The NASBA technique described here can detect three isolates of infuenza A subtype H5 and do not ..., Abstract
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Based on the reported Big liver and spleen disease virus (BLSV) gene sequence, a pair of primers was designed and a 375bp product was amplified from the total RNA extracted from homogenates of BLSV-infected livers. Twenty liver samples were randomly collected from two commercial broiler breeder ..., Abstract
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Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower ..., Abstract
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