Hello guest, Register  |  Sign In
Articles list with the same subject [Indirect ELISA] of Virologica Sinica
Full-length nucleoproteins from Ebola and Marburg viruses were expressed as His-tagged recombinant proteins in Escherichia coli and nucleoprotein-based enzyme-linked immunosorbent assays (ELISAs) were established for the detection of antibodies specifi c to Ebola and Marburg viruses. The ELISAs ..., Abstract
HTML Full Text  |  Free PDF (6662.70KB)  |  Springerlink
Porcine reproductive and respiratory syndrome is caused by the PRRS virus (PRRSV), which has six structural proteins (GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay (ELISA) and other methods. Toward this goal, we ..., Abstract
HTML Full Text  |  Free PDF (403.05KB)  |  Springerlink
In this study, a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV) E2 glycoprotein was expressed in E. coli to a obtain target protein. This target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV ..., Abstract
HTML Full Text  |  Free PDF (412.35KB)
The nucleocapsid protein (N) is a major structural protein of coronaviruses. The N protein of bat SARS-like coronavirus (SL-CoV) has a high similarity with that of SARS-CoV. In this study, the SL-CoV N protein was expressed in Escherichia coli, purified and used as antigen. An Indirect ..., Abstract
HTML Full Text  |  Free PDF (395.38KB)  |  Springerlink
Abstract: The sequence encoding an E2 main antigen glycoprotein of the C strain of classical swine fever virus (CSFV) was highly expressed in the host cell E. coli BL21–CodonPlus(DE3)–RIL using the pGEX-4T-1 expression vector and the soluble recombinant product purified with Glutathione Sepharose ..., Abstract
HTML Full Text  |  Free PDF (227.64KB)  |  Springerlink
The recombinant plasmid pET-VP_2Ⅰ was transformed into BL21 competent cells and expressed in high level after induced with 1.0mmol/l IPTG.The expressed product was purified with His·Bind chromatograghy after being proved by Westen-blot,and was used as an antigen to establish indirect ELISA for ..., Abstract
Free PDF (172.06KB)
The complete HA gene of AIV H9N2 subtype was amplified with PCR method using a pair of specific primers designed according to the relevant nucleotide sequence from GenBank. The PCR product was successively cloned into pMD18-T and pET-32a (+) to get a prokaryotic expression vector pET-HA. The target ..., Abstract
Free PDF (382.74KB)
The envelope glycoprotein E2 of classical swine fever virus(CSFV) was an important protective antigen with more than thirty hydrophobic amino acids at the carboxyl terminal. E2 genes with different lengths of transmembrane region(TMR) of Hog cholera lapinised virus(HCLV) were amplified by ..., Abstract
Free PDF (172.07KB)
E0 protein of Classical swine fever virus(CSFV) is of discriminatory feature in serum diagnostic test for developing CSFV Marker vaccine. It is also expected to be useful for monitoring CSFV prevalence,evaluating immunization efficacy and establishing immunization procedure. To obtain a large ..., Abstract
Free PDF (215.28KB)
The recombinant plasmid pETN was transformed into E.coli BL21 (DE3) host cell and the expression product-recombinant nucleocapsid protein of PRRSV was obtained under the optimized condition of host cell cultivation and IPTG induction. Consequently, the expression product was purified by means of ..., Abstract
Free PDF (588.08KB)
Torch is the abbreviation of four pathgenic microbols: Tonoplasma gondic (Toxo), Rubella Virus (RuV), Cytomegalovirus (CMV), and Herpes Simplex Virus (HSV 1 2 ). A new indirect ELISA reagent to detect the specific IgG and IgM antibody to ToRCH has been prepared. McAbs, labeled with horseradish ..., Abstract
Free PDF (184.91KB)
Up1Next20/Page, 1 Pages , Go to page  
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved