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Articles list with the same subject [RNA polymerase] of Virologica Sinica
The double-shelled grass carp reovirus (GCRV) is capable of endogenous RNA transcription and processing. Genome sequence analysis has revealed that the protein VP2, encoded by gene segment 2 (S2), is the putative RNA-dependent RNA polymerase (RdRp). In previous work, we have ex-pressed the ..., Abstract
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During transcription initiation, RNA polymerase binds tightly to the promoter DNA defining the start of transcription, transcribes comparatively slowly, and frequently releases short transcripts (3-8 nucleotides) in a process called abortive cycling. Transitioning to elongation, the second phase of ..., Abstract
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Baculoviruses encode a DNA-directed RNA polymerase that is evolutionarily divergent from cellular polymerases. This RNA polymerase is a multifunctional complex that has the ability to recognize late promoters, transcribe linked genes, and process transcripts at both the 5’ and 3’ ends. The LEF-4 ..., Abstract
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Baculoviruses are the only nuclear replicating DNA-containing viruses that encode their own DNA-directed RNA polymerase (RNAP). The baculovirus RNAP is specific for the transcription of genes expressed after virus DNA replication. It is composed of four subunits, making it the simplest multisubunit ..., Abstract
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he RdRp gene of Foot-and-mouth disease virus was amplified by reverse transcription polymerase chain reaction (RT-PCR) using pfuultraTM High-Fidelity DNA polymerase, and was then cloned into the prokaryotic expression vector pET-28a (+). E.coli BL-21 cells were transformed with the recombinant ..., Abstract
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A recombinant HCV RNA-dependent RNA polymerase (RdRp, NS5B protein) was expressed in E. coli strain BL21(DE3). The purified protein was used to investigate the RNA syntheses from the 3′ terminal sequences of HCV positive and negative strand RNA in vitro. The positive strand RNA did not direct the ..., Abstract
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Abstract:Grass carp reovirus(GCRV)is a disaster agent to aquatic animals,which belongs to genus
Aquareovirus.family Reoviridae.Sequences an alysis revealed GCRV S2 was 3877 nucleotides long
encoding a 1 38kDa protein VP2,which is deduced as virus RNA polymerase.To understan d the ..., Abstract
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A reovirus (designated as EsRV905), was isolated from Chinese Mitten Crab(Eriocheir
sinensis). Viral genome was extxacted with Trizol reagent and individual segment was recovered
separately from gel after 6.5% PAGE. The cDNA library of RNA1 was synthesized using random
primer ..., Abstract
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According to the genomic sequence of PLRV S, two pairs of specific primers were designed and synthesized. The cDNA of the replicase gene of PLRV Chinese isolate (PLRV Ch) was synthesized and amplified by RT PCR using the viral RNA as a template. The synthesized 3′ and 5′ cDNA fragments ..., Abstract
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RNA - dependent RNA polymerase extracted from the cucumber cotyledons four days afterinoculation by CMV-B-1 containing attenuation satellite RNA was used to initiate synthesis of(-)sense RNA from(+)sense RNA,using both CMV genomic or satellite RNAs as tem-plates.In presence of satellite RNA,the ..., Abstract
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