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Articles list with the same subject [VP2] of Virologica Sinica
Enterovirus 71 (EV71) infection causes severe central nervous system damage, particularly for children under the age of 5 years old, which remains a major public health burden worldwide. Clinical data released that children may be repeatedly infected by different members in enterovirus and get ..., Abstract
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The double-shelled grass carp reovirus (GCRV) is capable of endogenous RNA transcription and processing. Genome sequence analysis has revealed that the protein VP2, encoded by gene segment 2 (S2), is the putative RNA-dependent RNA polymerase (RdRp). In previous work, we have ex-pressed the ..., Abstract
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The gene encoding the VP28 envelope protein of White spot syndrome virus (WSSV) was cloned into expression vector pET-30a and transformed into the Escherichia coli strain BL21. After induction, the recombinant VP28 (rVP28) protein was purified and then used to immunize Balb/c mice for monoclonal ..., Abstract
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The herpes simplex virus type 1 (HSV-1) VP22, is one of the most abundant HSV-1 tegument proteins with an average stoichiometry of 2 400 copies per virion and conserved among alphaherpesvirinae. Many functions are attributed to VP22, including nuclear localization, chromatin binding, microtubule ..., Abstract
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A strain of canine parvovirus(CPV)was isolated from feces of an ill puppy in an animal hospital in Wuhan, China. It was designated as CPV/WH02/06. This isolate was identified as serotype CPV-2a by the hemagglutination test, CPV Ag detection strip, electron microscopy, and PCR. The vp2 gene was ..., Abstract
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We developed a sensitive and rapid lateral-flow immunoassay (LFIA) for WSSV, using a colloidal gold as an indicator. The fusion protein, VP (19+28), was expressed in E. coli, purified and used to prepare polyclonal antibodies. The purified anti-VP (19+28) IgG were conjugated with colloidal gold. ..., Abstract
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To study the specific subcellular localization of different regions of Pseudorabies virus (PrV) VP22, a series of mutants with the C-terminal truncations were amplified from a plasmid containing the full-length VP22 gene of the Ea strain. The resulting truncation constructs were fused to ORF ..., Abstract
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An expression cassette containing the VP2 gene of infectious bursal disease virus (IBDV) strain JS under the control of the CMV promoter and an enhancer in pcDNA3.1-VP2 was amplified by PCR and cloned into pUC18-US10 plasmid to yield the transferring vector pUC18-US10-VP2. The recombinant virus ..., Abstract
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A pair of primers was designed according to the sequence of vp28 gene of White spot syndrome virus (WSSV) in the GenBank. The vp28 DNA fragment was amplified by PCR and cloned into E. coli expression vector pET- 22b(+) successfully. Then pET22b-vp28 was transformed into E. coli. After IPTG ..., Abstract
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Viral protein VP22 is indispensable for the growth and replication of Marek’s disease virus serotype 1(MDV-1). In this study, vp22 gene was amplified from and avirluent CVI988/Rispens strain. Antibodies specific to the carboxyl terminus of VP22 expressed in E. coli were generated by immunizing ..., Abstract
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In this study, the open reading frame of PPV vp2 was amplified by PCR, sequenced, cloned and expressed both in prokaryotic and insect cells. In E. coli, by using pET-32(a) expression vector, VP2 was expressed as a Trx-His-S Tag fusion protein with the size of 84 kDa and the protein was used to ..., Abstract
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In order to construct a recombinant canine adenovirus type 2 (CAV-2) of the VP2 of Feline distemper virus or Feline panleukopenia virus (FPV), the vp2 gene fragment of FPV GT-2 strain was amplified by PCR and cloned into pVAX1 vector. The complete VP2 expression cassette was subcloned into the ..., Abstract
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The recombinant plasmid pET-VP_2Ⅰ was transformed into BL21 competent cells and expressed in high level after induced with 1.0mmol/l IPTG.The expressed product was purified with His·Bind chromatograghy after being proved by Westen-blot,and was used as an antigen to establish indirect ELISA for ..., Abstract
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A very virulent strain SD0210 of Infectious bursal disease virus (vvIBDV) was adapted to chicken embryo fibroblast cell (CEF) after propagating in SPF chicken embryo for 10 generations and CEF for 18 generations. The test of pathogenicity, virulent stability and immunogenicity in chickens showed ..., Abstract
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A 969bp fragment at the 5′- end of the vp2 gene of Goose parvovirus isolate HG5/82 was subcloned into the Nco I site of prokaryotic expression vector pPROEX~{TM}HTb. The recombinant plasmid was transformed into E.coli DH5α and induced with IPTG. SDS-PAGE analysis showed an induced product band ..., Abstract
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Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a ..., Abstract
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Hypervariable region of vp2 gene of six different IBDV (Infectious bursal disease virus) isolates, which were isolated from Jiangsu province, was amplified by RT-PCR and sequenced. Furthermore, sequences of six IBDV isolates were analyzed by ClustalX and Phylip3.5. It was suggested that three ..., Abstract
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Two pairs of primers were designed according to the sequences of envelope genes, vp19 and vp28 of White spot syndrome virus (WSSV) in the GenBank. The two DNA fragments about 370bp and 630bp amplified by PCR were linked to the EcoR I restriction endonuclease site and cloned into E.coli expression ..., Abstract
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Here we report that the major antigenic protein VP2 of very virulent Infectious bursal disease virus (vvIBDV) was secretively expressed with high level in yeast (Pichia pastoris) expression system. Primers were designed to amplify the previously cloned vvIBDV-vp2 gene from the plasmid pMD18-T- VP2. ..., Abstract
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vp2 gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced .The results showed that vp2 gene of Porcine parvovirus (PPV) SD-68 strain was 1740bp and encoded a protein of 579 amino acids. vp2 gene of SD-68 strain exhibited over 99% nucleotide sequence identity and 96% amino acid ..., Abstract
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