Citation: Jiaming Lan, Yao Deng, Jingdong Song, Baoying Huang, Wenling Wang, Wenjie Tan. Significant Spike-Specific IgG and Neutralizing Antibodies in Mice Induced by a Novel Chimeric Virus-Like Particle Vaccine Candidate for Middle East Respiratory Syndrome Coronavirus[J]. VIROLOGICA SINICA, 2018, 33 (5): 453-455 https://doi.org/10.1007/s12250-018-0064-8
Received: 28 August, 2018; Accepted: 11 October 2018; Published: 29 October 2018
Copyright: © Wuhan Institute of Virology, CAS and Springer Nature Singapore Pte Ltd. 2018
Data Availability: All relevant data are within the paper and its Supporting Information files.
Corresponding author: Wenjie Tan, E-mail: firstname.lastname@example.org.
Middle East respiratory syndrome coronavirus (MERSCoV), first isolated in 2012, has emerged zoonotically among humans (van Boheemen et al. 2012). Since then, MERS-CoV continues to be a public health concern, with a fatality rate of 35%. On-going MERS-CoV outbreaks highlight the urgent need for the development of interventional measures, including an effective vaccine against MERS-CoV infection. Currently, no licensed therapeutic or vaccine is available (Okba et al. 2017).
MERS-CoV spike (S) protein mediates coronaviral penetration into host cells via initial interaction with the host cell surface receptor. Based on the major immunodominant region of S protein, vaccines against MERSCoV infection have been developed using purified coronaviral S protein and DNA or viral vector-based vaccines expressing full-length MERS-CoV S protein or part of the S protein (Okba et al. 2017). However, efforts are required to achieve optimal protective responses, such as a higher titer of neutralizing antibody and protection against MERSCoV infection.
Virus-like particles (VLPs) are similar in size and morphology to intact virions, thereby rendering them ideal candidates as antigens or immunogens. Since VLPs cannot replicate in humans or revert to a pathogenic form, they are suitable candidates for vaccine development, especially those against life-threatening viruses such as SARS-CoV or MERS-CoV, which cause high morbidity and death. By coexpressing the S, envelope (E), and membrane (M) genes, Wang et al. generated VLPs of MERS-S (2017). However, it is difficult to generate these VLPs in large amounts owing to the lack of an efficient viral core protein. Chimeric VLPs (cVLPs) of SARS-CoV, containing the SARS S protein and the matrix protein of influenza virus using the baculovirus insect cell expression system, completely protected mice from death when administered intramuscularly or intranasally in the absence of an adjuvant (Liu et al. 2011). The use of influenza M1 as a core protein to generate cVLPs for other viruses is reportedly a promising approach, potentially increasing the yield and activating antigen-specific B cells for differentiation and antibody secretion (Liu et al. 2011). In the present study, we developed a novel type of cVLPs containing modified MERS-CoV S protein and the avian influenza matrix 1 (M1) protein, using a baculovirus insect cell expression system.
We first constructed a recombinant plasmid pFastBacDual-M1-St/HAk, using a similar strategy to that described previously (Liu et al. 2011), wherein a modified spike (St/HAk) of MERS-CoV (aa 1 to 1295 of MERS-CoV Spike, codon optimized DNA sequence based on Genbank:DQ420166.1, fused to aa 531-568 of H5N1 HA) and matrix protein 1 (M1) of A/Anhui/01/2005 (H5N1) (aa 1 to 758 of H5N1 M1 codon optimized DNA sequence based on GenBank: HM172159.1) were expressed, as shown in Fig. 1A. To construct the St/HAk, the transmembrane and carboxyl termini (TM/CT, aa 1296 to 1353 of MERS-CoV Spike) of MERS S protein were replaced with the corresponding segment (TM/CT) of HA-derived A/Anhui/01/2005 (H5N1). Thus, the recombinant St/HAk gene contained the ectodomain of MERS-CoV Spike and TM/CT of H5N1 HA. pFastBacDual-M1-St/HAk was digested with restriction enzymes KpnⅠ and NheⅠ. Further sequenced to ensure the accuracy of the sequences of M1 and St/HAk. The tandem chimeric S/M1 plasmid pFastBacDual-M1-St/HAk was transformed into competent cells of DH10BacTM (Invitrogen, California, USA) to generate recombinant bacmids (Bacmid M1-St/HAk), and verified via PCR. Purified recombinant bacmid DNA was transfected into Sf9 cells (ATCC CRL-1711) to generate recombinant baculoviral M1-St/HAk, using a Bac-to-Bac baculoviral expression system (Invitrogen). Harvested Sf9 cells infected with baculovirus M1-St/HAk were lysed with SDSPAGE buffer. Recombinant proteins were identified via Western blot analysis and indirect immunofluorescence (Lan et al. 2014; Deng et al. 2018).
Thereafter, we harvested the P3 stock of recombinant baculovirus M1-St/HAk from infected Sf9 cells at a titer of 8 × 107 PFU/mL. Suspended High FiveTM cells were infected by recombinant baculovirus M1-St/HAk at a multiplicity of infection (MOI) of 1, 5, and 10, and harvested at different time points (24, 72, and 96 h). Protein expression was analyzed via SDS-PAGE and Western blot analysis to determine the optimal conditions. Recombinant M1-St/HAk protein expression was highest at a MOI of 5 after 72 h. After large-scale amplification, the recombinant proteins yielded bands at approximately ~ 157 kDa, which represents a monomer of the S protein ectodomain, a ~ 110-kDa band, which represents the cleavage of S glycoprotein into an amino-terminal domain (S1), and a 25-kDa band, which represents the M1 protein of H5N1 (Fig. 1B), thereby confirming MERS-CoV S protein and H5N1 M1 protein expression. After purification via ultracentrifugation at 4 ℃, 3, 000 rpm for 2 h, the cVLPs of MERS-S were negatively stained and observed via transmission electron microscope. Enveloped VLPs displayed a spheroid morphology, with a diameter of 80-100 nm and displayed morphological similarity with CoV virions, with enveloped spikes arranged in a crown shape (Fig. 1C-a). After labelling with murine monoclonal antibodies against S protein of MERS-CoV and gold-labeled goat anti-mouse IgG antibody, S specific proteins shown as black pellets were observed on the cVLPS (Fig. 1C-b). These results indicate that chimeric MERS VLPs are morphologically similar to native MERS-CoV (https://www.cdc.gov/coronavirus/mers/photos.html).
To assess the immunogenicity of cVLPS in mice, 6–8-week-old female BALB/c mice were intramuscularly injected with 1 lg of cVLPs of MERS-S adjuvanted with 100 lL of aluminum (A) and 10 lg of CpG (C). Simultaneously, the mice of control group inoculated with adjutants (A + C) only. All mice were immunized thrice at 4-week intervals. Mice were bled via the venae angularis and serum was harvested from whole blood to determine IgG levels via enzyme-linked immunosorbent assay (ELISA) and neutralization activity was assessed via the pseudovirus neutralization assay for MERS-CoV (Lan et al. 2014; Deng et al. 2018) As shown in Fig. 1D, 2 weeks after the second immunization, the total S proteinspecific IgG titer approached 1:80, 000 in the VLP group, being significantly higher than that in the control group. After the third immunization, the IgG titer was further elevated (more than 105). More importantly, high titers of neutralization antibodies (at a serum dilution of 1:320, > 50% neutralizing activity based on the pseudovirus neutralization assay, indicated as pI50 = 320) were also detected in mice immunized with cVLPs (Fig. 1E).
We developed immunogenic cVLPs of MERS-S via coexpression of H5N1 M1 protein and MERS-CoV S protein in a baculoviral expression system. The present results indicate that cVLPs with surface expression of MERS-CoV S protein, morphologically emulating the native virus, are promising candidates for prophylactic vaccines for MERSCoV infections. A similar strategy was adopted to formulate cVLPs of SARS-CoV (Liu et al. 2011). The present results thus indicate that cVLPs of MERS-S mimic wild type viral morphology and induce robust S-specific humoral immune responses in mice. In the future study, we intend to use a similar platform to develop cVLPs of other CoVs or highly pathogenic viruses to further validate the present strategy and to purify cVLPs of MERS-S on a large scale to evaluate the protective effect of cVLPs in animal models of infection.
This work was supported by the Megaproject for Infectious Disease Research of China (2016ZX10004001-003 and 2014ZX10004001-002 to W.T.), and the National Key Research and Development Program of China (2016YFD0500300 to W.T., 2016YFC1200901 to Y.D., and 2016YFC1200200 to B.H). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Animal and Human Rights Statement
Animal studies were conducted in strict accordance with the Guide for the Care and Use of Laboratory Animals of the People's Republic of China. The study protocol was approved by the Committee on the Ethics of Animal Experiments of the Chinese Centre for Disease Control and Prevention.
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