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Volume 29 Issue 5
October 2014
    Citation: Tetiana Krupodorova, Svetlana Rybalko, Victor Barshteyn. Antiviral activity of Basidiomycete mycelia against influenza type A (serotype H1N1) and herpes simplex virus type 2 in cell culture .VIROLOGICA SINICA, 2014, 29(5) : 284-290.  http://dx.doi.org/10.1007/s12250-014-3486-y
    shu

    Antiviral activity of Basidiomycete mycelia against influenza type A (serotype H1N1) and herpes simplex virus type 2 in cell culture

    cstr: 32224.14.s12250-014-3486-y
    • 1.

      Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, Kyiv 04123, Ukraine

    • 2.

      Institute of Epidemiology and Infectious Diseases of the Academy of Medical Sciences of Ukraine, Kyiv 03038, Ukraine

    • Corresponding author: Victor Barshteyn, barmash14@gmail.com
    • Received Date: 03 July 2014
      Accepted Date: 15 October 2014
      Published Date: 24 October 2014
      Available online: 01 October 2014
    • In this study, we investigated the in vitro antiviral activity of the mycelia of higher mushrooms against influenza virus type A (serotype H1N1) and herpes simplex virus type 2 (HSV-2), strain BH. All 10 investigated mushroom species inhibited the reproduction of influenza virus strain A/FM/1/47 (H1N1) in MDCK cells reducing the infectious titer by 2.0-6.0 lg ID50. Four species, Pleurotus ostreatus, Fomes fomentarius, Auriporia aurea, and Trametes versicolor, were also determined to be effective against HSV-2 strain BH in RK-13 cells, with similar levels of inhibition as for influenza. For some of the investigated mushroom species—Pleurotus eryngii, Lyophyllum shimeji, and Flammulina velutipes—this is the first report of an anti-influenza effect. This study also reports the first data on the medicinal properties of A. aurea, including anti-influenza and antiherpetic activities. T. versicolor 353 mycelium was found to have a high therapeutic index (324.67), and may be a promising material for the pharmaceutical industry as an anti-influenza and antiherpetic agent with low toxicity. Mycelia with antiviral activity were obtained in our investigation by bioconversion of agricultural wastes (amaranth flour after CO2 extraction), which would reduce the cost of the final product and solve some ecological problems.
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      Antiviral activity of Basidiomycete mycelia against influenza type A (serotype H1N1) and herpes simplex virus type 2 in cell culture

        Corresponding author: Victor Barshteyn, barmash14@gmail.com
      • 1. Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, Kyiv 04123, Ukraine
      • 2. Institute of Epidemiology and Infectious Diseases of the Academy of Medical Sciences of Ukraine, Kyiv 03038, Ukraine
      • Received Date: 03 July 2014
      • Accepted Date: 15 October 2014
      • Published Date: 24 October 2014

      Abstract: In this study, we investigated the in vitro antiviral activity of the mycelia of higher mushrooms against influenza virus type A (serotype H1N1) and herpes simplex virus type 2 (HSV-2), strain BH. All 10 investigated mushroom species inhibited the reproduction of influenza virus strain A/FM/1/47 (H1N1) in MDCK cells reducing the infectious titer by 2.0-6.0 lg ID50. Four species, Pleurotus ostreatus, Fomes fomentarius, Auriporia aurea, and Trametes versicolor, were also determined to be effective against HSV-2 strain BH in RK-13 cells, with similar levels of inhibition as for influenza. For some of the investigated mushroom species—Pleurotus eryngii, Lyophyllum shimeji, and Flammulina velutipes—this is the first report of an anti-influenza effect. This study also reports the first data on the medicinal properties of A. aurea, including anti-influenza and antiherpetic activities. T. versicolor 353 mycelium was found to have a high therapeutic index (324.67), and may be a promising material for the pharmaceutical industry as an anti-influenza and antiherpetic agent with low toxicity. Mycelia with antiviral activity were obtained in our investigation by bioconversion of agricultural wastes (amaranth flour after CO2 extraction), which would reduce the cost of the final product and solve some ecological problems.

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