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Volume 34 Issue 1
February 2019
    Citation: Juemin Xi, Fei Ye, Guanzhou Wang, Wei Han, Zhizhong Wei, Bin Yin, Jiangang Yuan, Boqin Qiang, Xiaozhong Peng. Polypyrimidine Tract-Binding Protein Regulates Enterovirus 71 Translation Through Interaction with the Internal Ribosomal Entry Site .VIROLOGICA SINICA, 2019, 34(1) : 66-67.  http://dx.doi.org/10.1007/s12250-019-00089-1
    shu

    Polypyrimidine Tract-Binding Protein Regulates Enterovirus 71 Translation Through Interaction with the Internal Ribosomal Entry Site

    • 1.

      Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, China

    • 2.

      The State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China

    • Corresponding author: Xiaozhong Peng, pengxiaozhong@pumc.edu.cn, ORCID: http://orcid.org/0000-0002-9592-9554
    • Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12250-019-00089-1) contains supplementary material, which is available to authorized users
      Juemin Xi and Fei Ye contributed equally to this work
    • Received Date: 03 September 2018
      Accepted Date: 19 December 2018
      Published Date: 22 February 2019
      Available online: 01 February 2019
    • Enterovirus 71 (EV71), a major causative agent of hand, foot, and mouth disease, has caused periodic infection outbreaks in children in the Asia–Pacific region. In order to describe the largely unknown life cycle of EV71, the molecular basis of its virus-host interactions must first be determined. The 50 untranslated region of EV71 contains a cloverleaf-like structure and internal ribosomal entry site (IRES), which play an important role in transcription and translation of viral protein. We found that polypyrimidine tract-binding protein 1 (PTB) bound to the IRES of EV71. RNA recognition motifs 1 and 2 of PTB were responsible for its binding to the EV71 IRES. Moreover, PTB protein was shuttled from nucleus to cytoplasm after EV71 infection. Additionally, IRES activity and viral protein production were inhibited by PTB knockdown. These results suggest that PTB interacts with the EV71 IRES, and positively regulates viral protein translation.
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      Polypyrimidine Tract-Binding Protein Regulates Enterovirus 71 Translation Through Interaction with the Internal Ribosomal Entry Site

        Corresponding author: Xiaozhong Peng, pengxiaozhong@pumc.edu.cn
      • 1. Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming 650118, China
      • 2. The State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China
      • Received Date: 03 September 2018
      • Accepted Date: 19 December 2018
      • Published Date: 22 February 2019

      Abstract: Enterovirus 71 (EV71), a major causative agent of hand, foot, and mouth disease, has caused periodic infection outbreaks in children in the Asia–Pacific region. In order to describe the largely unknown life cycle of EV71, the molecular basis of its virus-host interactions must first be determined. The 50 untranslated region of EV71 contains a cloverleaf-like structure and internal ribosomal entry site (IRES), which play an important role in transcription and translation of viral protein. We found that polypyrimidine tract-binding protein 1 (PTB) bound to the IRES of EV71. RNA recognition motifs 1 and 2 of PTB were responsible for its binding to the EV71 IRES. Moreover, PTB protein was shuttled from nucleus to cytoplasm after EV71 infection. Additionally, IRES activity and viral protein production were inhibited by PTB knockdown. These results suggest that PTB interacts with the EV71 IRES, and positively regulates viral protein translation.

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