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Volume 36 Issue 2
April 2021
    Citation: Dan Li, Yinguang Liu, Xiaolan Qi, Yuan Wen, Pan Li, Zhao Ma, Yongjie Liu, Haixue Zheng, Zhijie Liu. African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs .VIROLOGICA SINICA, 2021, 36(2) : 187-195.  http://dx.doi.org/10.1007/s12250-021-00350-6
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    African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs

    cstr: 32224.14.s12250-021-00350-6

    • State Key Laboratory of Veterinary Etiological Biology and OIE/National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

    • Corresponding author: Haixue Zheng, haixuezheng@163.com, ORCID: 0000-0001-6850-1379
      Zhijie Liu, liuzhijie@caas.cn, ORCID: 0000-0002-1895-6452
    • Dan Li, Yinguang Liu and Xiaolan Qi contributed equally to this work.
    • Received Date: 18 July 2020
      Accepted Date: 17 December 2020
      Published Date: 10 March 2021
      Available online: 01 April 2021

    • African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), an often lethal disease in domestic and wild pigs. ASF represents a major threat to the swine industry worldwide. Currently, no commercial vaccine is available because of the complexity of ASFV or biosecurity concerns. Live attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain challenge. In the present study, a mutant ASFV strain with the deletion of ASFV MGF-110-9L (ASFV-Δ9L) was generated from a highly virulent ASFV CN/GS/2018 parental strain, a genotype II ASFV. Relative to the parental ASFV isolate, deletion of the MGF-110-9L gene significantly decreased the ability of ASFV-Δ9L to replicate in vitro in primary swine macrophage cell cultures. The majority of animals inoculated intramuscularly with a low dose of ASFV-Δ9L (10 HAD50) remained clinically normal during the 21-day observational period. Three of five ASFV-Δ9L-infected animals displayed low viremia titers and low virus shedding and developed a strong virus-specific antibody response, indicating partial attenuation of the ASFV-Δ9L strain in pigs. The findings imply the potential usefulness of the ASFV-Δ9L strain for further development of ASF control measures.

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      African Swine Fever Virus MGF-110-9L-deficient Mutant Has Attenuated Virulence in Pigs

        Corresponding author: Haixue Zheng, haixuezheng@163.com
        Corresponding author: Zhijie Liu, liuzhijie@caas.cn
      • State Key Laboratory of Veterinary Etiological Biology and OIE/National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
      • Received Date: 18 July 2020
      • Accepted Date: 17 December 2020
      • Published Date: 10 March 2021

      Abstract: 

      African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), an often lethal disease in domestic and wild pigs. ASF represents a major threat to the swine industry worldwide. Currently, no commercial vaccine is available because of the complexity of ASFV or biosecurity concerns. Live attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain challenge. In the present study, a mutant ASFV strain with the deletion of ASFV MGF-110-9L (ASFV-Δ9L) was generated from a highly virulent ASFV CN/GS/2018 parental strain, a genotype II ASFV. Relative to the parental ASFV isolate, deletion of the MGF-110-9L gene significantly decreased the ability of ASFV-Δ9L to replicate in vitro in primary swine macrophage cell cultures. The majority of animals inoculated intramuscularly with a low dose of ASFV-Δ9L (10 HAD50) remained clinically normal during the 21-day observational period. Three of five ASFV-Δ9L-infected animals displayed low viremia titers and low virus shedding and developed a strong virus-specific antibody response, indicating partial attenuation of the ASFV-Δ9L strain in pigs. The findings imply the potential usefulness of the ASFV-Δ9L strain for further development of ASF control measures.

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