Citation: Weijie Wang, Jinxuan Yang, Pinfang Kang, Jinsong Bai, Xiaoli Feng, Liuqi Huang, Yiming Zhang, Yuechun Wu, Bi Tang, Hongju Wang, JianJie Jiang, Minghua Li, Bing Zhao, Xinglou Yang. Direct infection of SARS-CoV-2 in human iPSC-derived 3D cardiac organoids recapitulates COVID-19 myocarditis .VIROLOGICA SINICA, 2023, 38(6) : 971-974.  http://dx.doi.org/10.1016/j.virs.2023.09.005

Direct infection of SARS-CoV-2 in human iPSC-derived 3D cardiac organoids recapitulates COVID-19 myocarditis

  • Highlights
    1. Establishment of 3D cardiac organoids composed of cardiomyocyte and endothelial layers.
    2. SARS-CoV-2 infection causes multi-lineage cardiac injuries.
    3. Cardiovascular health should be greatly concerned in COVID-19 patients.

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    1. Bailey, A.L., Dmytrenko, O., Greenberg, L., Bredemeyer, A.L., Ma, P., Liu, J., Penna, V., Winkler, E.S., Sviben, S., Brooks, E., Nair, A.P., Heck, K.A., Rali, A.S., Simpson, L., Saririan, M., Hobohm, D., Stump, W.T., Fitzpatrick, J.A., Xie, X., Zhang, X., Shi, P.Y., Hinson, J.T., Gi, W.T., Schmidt, C., Leuschner, F., Lin, C.Y., Diamond, M.S., Greenberg, M.J., Lavine, K.J., 2021. SARS-CoV-2 infects human engineered heart tissues and models COVID-19 myocarditis. JACC Basic Transl Sci 6, 331–345.

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    4. Bräuninger, H., Stoffers, B., Fitzek, A.D.E., Meißner, K., Aleshcheva, G., Schweizer, M., Weimann, J., Rotter, B., Warnke, S., Edler, C., Braun, F., Roedl, K., Scherschel, K., Escher, F., Kluge, S., Huber, T.B., Ondruschka, B., Schultheiss, H.P., Kirchhof, P., Blankenberg, S., Püschel, K., Westermann, D., Lindner, D., 2022. Cardiac SARS-CoV-2 infection is associated with pro-inflammatory transcriptomic alterations within the heart. Cardiovasc. Res. 118, 542–555.

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    9. Mills, R.J., Humphrey, S.J., Fortuna, P.R.J., Lor, M., Foster, S.R., Quaife-Ryan, G.A., Johnston, R.L., Dumenil, T., Bishop, C., Rudraraju, R., Rawle, D.J., Le, T., Zhao, W., Lee, L., Mackenzie-Kludas, C., Mehdiabadi, N.R., Halliday, C., Gilham, D., Fu, L., Nicholls, S.J., Johansson, J., Sweeney, M., Wong, N.C.W., Kulikowski, E., Sokolowski, K.A., Tse, B.W.C., Devilée, L., Voges, H.K., Reynolds, L.T., Krumeich, S., Mathieson, E., Abu-Bonsrah, D., Karavendzas, K., Griffen, B., Titmarsh, D., Elliott, D.A., McMahon, J., Suhrbier, A., Subbarao, K., Porrello, E.R., Smyth, M.J., Engwerda, C.R., MacDonald, K.P.A., Bald, T., James, D.E., Hudson, J.E., 2021. BET inhibition blocks inflammation-induced cardiac dysfunction and SARS-CoV-2 infection. Cell 184, 2167–2182.e22.

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    13. Schurink, B., Roos, E., Radonic, T., Barbe, E., Bouman, C.S.C., de Boer, H.H., de Bree, G.J., Bulle, E.B., Aronica, E.M., Florquin, S., Fronczek, J., Heunks, L.M.A., de Jong, M.D., Guo, L., du Long, R., Lutter, R., Molenaar, P.C.G., Neefjes-Borst, E.A., Niessen, H.W.M., van Noesel, C.J.M., Roelofs, J.J.T.H., Snijder, E.J., Soer, E.C., Verheij, J., Vlaar, A.P.J., Vos, W., van der Wel, N.N., van der Wal, A.C., van der Valk, P., Bugiani, M., 2020. Viral presence and immunopathology in patients with lethal COVID-19: a prospective autopsy cohort study. Lancet. Microbe. 1, e290–e299.

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    Direct infection of SARS-CoV-2 in human iPSC-derived 3D cardiac organoids recapitulates COVID-19 myocarditis

      Corresponding author: Pinfang Kang, kangpinfang.1016@163.com
      Corresponding author: Bing Zhao, bingzhao@fudan.edu.cn
      Corresponding author: Xinglou Yang, yangxinglou@mail.kiz.ac.cn
    • a. Department of Cardiovascular Disease, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, China;
    • b. Yunnan Key Laboratory of Biodiversity Information, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650201, China;
    • c. State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China;
    • d. Kunming Third People's Hospital, Kunming, 650041, China;
    • e. Kunming National High-level Biosafety Research Center for Non-human Primates, Kunming Institute of Zoology, Chinese Academic of Sciences, Kunming, 650107, China;
    • f. Institute of Organoid Technology, Kunming Medical University, Kunming, 650500, China

    Abstract: Highlights
    1. Establishment of 3D cardiac organoids composed of cardiomyocyte and endothelial layers.
    2. SARS-CoV-2 infection causes multi-lineage cardiac injuries.
    3. Cardiovascular health should be greatly concerned in COVID-19 patients.

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