Recombinant human interferon-α1b inhibits SARS-CoV-2 better than interferon-α2b <i>in vitro</i>

Danrong Shi; Keda Chen; Xiangyun Lu; Linfang Cheng; Tianhao Weng; Fumin Liu; Nanping Wu; Lanjuan Li; Hangping Yao

doi:10.1016/j.virs.2022.01.031

April 2022

Citation: Danrong Shi, Keda Chen, Xiangyun Lu, Linfang Cheng, Tianhao Weng, Fumin Liu, Nanping Wu, Lanjuan Li, Hangping Yao. Recombinant human interferon-α1b inhibits SARS-CoV-2 better than interferon-α2b in vitro .VIROLOGICA SINICA, 2022, 37(2) : 295-298. http://dx.doi.org/10.1016/j.virs.2022.01.031

Recombinant human interferon-α1b inhibits SARS-CoV-2 better than interferon-α2b in vitro

Danrong Shi ^a ,
Keda Chen ^b ,
Xiangyun Lu ^a ,
Linfang Cheng ^a ,
Tianhao Weng ^a ,
Fumin Liu ^a ,
Nanping Wu ^{a
,,} ,
Lanjuan Li ^{a
,,} ,
Hangping Yao ^{a
,,}

a State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China
b Shulan International Medical College, Zhejiang Shuren University, Hangzhou, 310015, China

Corresponding author: Nanping Wu, flwnp2013@163.com
Lanjuan Li, ljli@zju.edu.cn
Hangping Yao, yaohangping@zju.edu.cn
Received Date: 20 February 2021
Accepted Date: 21 January 2022
Available online: 25 January 2022

Abstract

Highlights
1) A comprehensive evaluation method for anti-SARS-CoV-2 drugs was established based on RT-qPCR, TCID₅₀ method, and immunofluorescence.
2) A significant antiviral effect of rHuIFN-α1b was shown with EC₅₀=0.12 IU/mL in Vero cells and EC₅₀=0.52 IU/mL in Calu-3 cells, which was better than rHuIFN-α2b (EC₅₀=0.25 IU/mL in Vero cells and EC₅₀=2.48 IU/mL in Calu-3 cells).
3) rHuIFN-α1b has a good potential in the application of anti-COVID-19 therapy.

Electronic Supplementary Material

10.1016j.virs.2022.01.031-ESM.docx
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