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Citation: Anamika Mishra, Muhasin Asaf, Amod Kumar, Diwakar Dattatreya Kulkarni, Richa Sood, Sandeep Bhatia, Bharat Bhushan, Ashwin Ashok Raut. Differential miRNA expression profiling of highly pathogenic avian influenza virus H5N1 infected chicken lungs reveals critical microRNAs, biological pathways and genes involved in the molecular pathogenesis [J].VIROLOGICA SINICA, 2022, 37(3) : 465-468.  http://dx.doi.org/10.1016/j.virs.2022.03.004

Differential miRNA expression profiling of highly pathogenic avian influenza virus H5N1 infected chicken lungs reveals critical microRNAs, biological pathways and genes involved in the molecular pathogenesis

  • Corresponding author: Anamika Mishra, reach2anamika@yahoo.com
  • Received Date: 24 January 2021
    Accepted Date: 07 March 2022
    Available online: 09 March 2022
  • Highlights
    · Highthrouput sequencing of small RNA of H5N1 infected and mock infected chicken lungs.
    · 297 miRNAs identified in mock-infected and 201 miRNAs identified in AIV infected chicken lungs.
    · 36 miRNAs were upregulated and 90 were downregulated during H5N1 infection.
    · Functional analysis and gene ontology of predicted target genes of expressed miRNAs.
    · MAPK pathway, NF-κB, IGF and gga-let-7b might play important role during H5N1 pathogenesis.

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    Differential miRNA expression profiling of highly pathogenic avian influenza virus H5N1 infected chicken lungs reveals critical microRNAs, biological pathways and genes involved in the molecular pathogenesis

      Corresponding author: Anamika Mishra, reach2anamika@yahoo.com
    • a Pathogenomics Laboratory, ICAR-National Institute of High Security Animal Diseases, OIE referral laboratory for Avian Influenza, Bhopal 462 021, India;
    • b ICAR-National Institute of High Security Animal Diseases, OIE referral lab for Avian Influenza, Bhopal 462 021, India;
    • c Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Bareilly 243 122, India

    Abstract: Highlights
    · Highthrouput sequencing of small RNA of H5N1 infected and mock infected chicken lungs.
    · 297 miRNAs identified in mock-infected and 201 miRNAs identified in AIV infected chicken lungs.
    · 36 miRNAs were upregulated and 90 were downregulated during H5N1 infection.
    · Functional analysis and gene ontology of predicted target genes of expressed miRNAs.
    · MAPK pathway, NF-κB, IGF and gga-let-7b might play important role during H5N1 pathogenesis.

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