Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

Yuanzhi Chen; Chenguang Shen; Jing Chen; Junyu Chen; Fentian Chen; Limin Zhang; Xue Liu; Siyuan Chen; Sen Xue; Yongliang Liu; Jixian Tang; Quan Yuan; Yixin Chen; Wenxin Luo; Ningshao Xia

doi:10.1016/j.virs.2022.03.009

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Volume 37 Issue 4

August 2022

Citation: Yuanzhi Chen, Chenguang Shen, Jing Chen, Junyu Chen, Fentian Chen, Limin Zhang, Xue Liu, Siyuan Chen, Sen Xue, Yongliang Liu, Jixian Tang, Quan Yuan, Yixin Chen, Wenxin Luo, Ningshao Xia. Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells .VIROLOGICA SINICA, 2022, 37(4) : 619-622. http://dx.doi.org/10.1016/j.virs.2022.03.009

Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

Yuanzhi Chen ^a ,
Chenguang Shen ^{a,b
,,} ,
Jing Chen ^a ,
Junyu Chen ^a ,
Fentian Chen ^a ,
Limin Zhang ^a ,
Xue Liu ^a ,
Siyuan Chen ^a ,
Sen Xue ^a ,
Yongliang Liu ^a ,
Jixian Tang ^a ,
Quan Yuan ^a ,
Yixin Chen ^{a
,,} ,
Wenxin Luo ^{a
,,} ,
Ningshao Xia ^a

a National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China;

Corresponding author: Chenguang Shen, a124965468@smu.edu.cn
Yixin Chen, yxchen2008@xmu.edu.cn
Wenxin Luo, wxluo@xmu.edu.cn
Received Date: 21 September 2021
Available online: 22 March 2022

Abstract

Highlights
1. Class-switch recombination was mimicked in hybridomas through a controllable expression system of activation-induced cytidine deaminase.
2. IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
3. IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo.

Electronic Supplementary Material

10.1016j.virs.2022.03.009-ESM.docx
References
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Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

Corresponding author: Chenguang Shen, a124965468@smu.edu.cn

Corresponding author: Yixin Chen, yxchen2008@xmu.edu.cn

Corresponding author: Wenxin Luo, wxluo@xmu.edu.cn

a National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China;

Received Date: 21 September 2021

Abstract: Highlights
1. Class-switch recombination was mimicked in hybridomas through a controllable expression system of activation-induced cytidine deaminase.
2. IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
3. IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo.