Taurolidine improved protection against highly pathogenetic avian influenza H5N1 virus lethal-infection in mouse model by regulating the NF-κB signaling pathway

Chaoxiang Lv; Yuanguo Li; Tiecheng Wang; Qiqi Zhang; Jing Qi; Mingwei Sima; Entao Li; Tian Qin; Zhuangzhuang Shi; Fangxu Li; Xuefeng Wang; Weiyang Sun; Na Feng; Songtao Yang; Xianzhu Xia; Ningyi Jin; Yifa Zhou; Yuwei Gao

doi:10.1016/j.virs.2022.11.010

February 2023

Citation: Chaoxiang Lv, Yuanguo Li, Tiecheng Wang, Qiqi Zhang, Jing Qi, Mingwei Sima, Entao Li, Tian Qin, Zhuangzhuang Shi, Fangxu Li, Xuefeng Wang, Weiyang Sun, Na Feng, Songtao Yang, Xianzhu Xia, Ningyi Jin, Yifa Zhou, Yuwei Gao. Taurolidine improved protection against highly pathogenetic avian influenza H5N1 virus lethal-infection in mouse model by regulating the NF-κB signaling pathway .VIROLOGICA SINICA, 2023, 38(1) : 119-127. http://dx.doi.org/10.1016/j.virs.2022.11.010

Taurolidine improved protection against highly pathogenetic avian influenza H5N1 virus lethal-infection in mouse model by regulating the NF-κB signaling pathway

Chaoxiang Lv ^a,b ,
Yuanguo Li ^b,c ,
Tiecheng Wang ^b ,
Qiqi Zhang ^a ,
Jing Qi ^a,b ,
Mingwei Sima ^b,d ,
Entao Li ^b ,
Tian Qin ^a,b ,
Zhuangzhuang Shi ^b,e ,
Fangxu Li ^b,f ,
Xuefeng Wang ^b ,
Weiyang Sun ^b ,
Na Feng ^b ,
Songtao Yang ^b ,
Xianzhu Xia ^b ,
Ningyi Jin ^{a,b,d,e
,,} ,
Yifa Zhou ^{a
,,} ,
Yuwei Gao ^{b,d,e,f
,,}

a. College of Life Sciences, Northeast Normal University, Changchun, Jilin, 130021, China;
b. Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Changchun, 130122, China;
c. College of Animal Medicine, Jilin University, Changchun, 130000, China;
d. College of Basic Medicine, Changchun University of Chinese Medicine, Changchun, 130117, China;
e. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130033, China;
f. College of Life Sciences, Shandong Normal University, Jinan, 250014, China

Corresponding author: Ningyi Jin, ningyik@126.com
Yifa Zhou, zhouyf383@nenu.edu.cn
Yuwei Gao, gaoyuwei@gmail.com
Received Date: 08 July 2021
Accepted Date: 21 November 2022
Available online: 28 November 2022

Abstract

Taurolidine (TRD), a derivative of taurine, has anti-bacterial and anti-tumor effects by chemically reacting with cell-walls, endotoxins and exotoxins to inhibit the adhesion of microorganisms. However, its application in antiviral therapy is seldom reported. Here, we reported that TRD significantly inhibited the replication of influenza virus H5N1 in MDCK cells with the half-maximal inhibitory concentration (EC₅₀) of 34.45 μg/mL. Furthermore, the drug inhibited the amplification of the cytokine storm effect and improved the survival rate of mice lethal challenged with H5N1 (protection rate was 86%). Moreover, TRD attenuated virus-induced lung damage and reduced virus titers in mice lungs. Administration of TRD reduced the number of neutrophils and increased the number of lymphocytes in the blood of H5N1 virus-infected mice. Importantly, the drug regulated the NF-κB signaling pathway by inhibiting the separation of NF-κB and IκBa, thereby reducing the expression of inflammatory factors. In conclusion, our findings suggested that TRD could act as a potential anti-influenza drug candidate in further clinical studies.
- Influenza viruses
- , H5N1
- , Taurolidine (TRD)
- , Cytokine storms
- , Inflammatory response
- , NF-κB signaling pathway

Electronic Supplementary Material

10.1016j.virs.2022.11.010-ESM.docx
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