Citation: Dong-Sheng Luo, Zhi-Jian Zhou, Xing-Yi Ge, Hervé Bourhy, Efstathios S. Giotis, Marc Grandadam, Zheng-Li Shi, Laurent Dacheux. Genomic characterization of three unclassified rhabdoviruses from mosquitoes in Malaysia and Central Africa .VIROLOGICA SINICA, 2025, 40(6) : 1045-1049.  http://dx.doi.org/10.1016/j.virs.2025.11.003

Genomic characterization of three unclassified rhabdoviruses from mosquitoes in Malaysia and Central Africa

  • Highlights
    1. Characterisation of three new species of insect-related rhabdoviruses based on coding-complete genome sequences.
    2. Identification of numerous putative genes in addition to the five canonical genes associated with rhabdoviruses.
    3. Demonstration that Porton virus (PORV) and Bangoran virus (BGNV) represent new species within the genus Hapavirus.
    4. Demonstration that Boteke virus (BOTV) is a new species in the genus Sunrhavirus.
    5. Improved knowledge of the genetic diversity of rhabdoviruses, particularly those that are potential arboviruses.

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    1. Allison, A.B., Mead, D.G., Palacios, G.F., Tesh, R.B., Holmes, E.C., 2014. Gene duplication and phylogeography of North American members of the Hart Park serogroup of avian rhabdoviruses. Virology 448, 284-292.

    2. Calisher, C.H., Karabatsos, N., Zeller, H., Digoutte, J.-P., Tesh, R.B., Shope, R.E., Rosa, A.P.A.T. da, George, T.D.S., 1989. Antigenic Relationships among Rhabdoviruses from Vertebrates and Hematophagous Arthropods. INT 30, 241-257.

    3. Kuhn, J.H., Abe, J., Adkins, S., Alkhovsky, S.V., Avsic-Zupanc, T., Ayllon, M.A., Bahl, J., Balkema-Buschmann, A., Ballinger, M.J., Kumar Baranwal, et al., 2023. Annual (2023) taxonomic update of RNA-directed RNA polymerase-encoding negative-sense RNA viruses (realm Riboviria: kingdom Orthornavirae: phylum Negarnaviricota). Journal of General Virology 104, 001864.

    4. Li, C.-X., Shi, M., Tian, J.-H., Lin, X.-D., Kang, Y.-J., Chen, L.-J., Qin, X.-C., Xu, J., Holmes, E.C., Zhang, Y.-Z., 2015. Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses. eLife 4, e05378.

    5. Longdon, B., Murray, G.G., Palmer, W.J., Day, J.P., Parker, D.J., Welch, J.J., Obbard, D.J., Jiggins, F.M., 2015. The evolution, diversity, and host associations of rhabdoviruses. Virus evolution 1, vev014.

    6. Luo, D.-S., Zhou, Z.-J., Ge, X.-Y., Bourhy, H., Shi, Z.-L., Grandadam, M., Dacheux, L., 2021. Genome Characterization of Bird-Related Rhabdoviruses Circulating in Africa. Viruses 13, 2168.

    7. McAllister, J., Gauci, P.J., Mitchell, I.R., Boyle, D.B., Bulach, D.M., Weir, R.P., Melville, L.F., Davis, S.S., Gubala, A.J., 2014. Genomic characterisation of Almpiwar virus, Harrison Dam virus and Walkabout Creek virus; three novel rhabdoviruses from northern Australia. Virology Reports 3-4, 1-17.

    8. Mekki, A.A.E., Nieuwenhuysen, P., van der Groen, G., Pattyn, S.R., 1981. Characterization of some ungrouped viruses. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 799-806.

    9. Walker, P.J., Dietzgen, R.G., Joubert, D.A., Blasdell, K.R., 2011. Rhabdovirus accessory genes. Virus Research 162, 110-125.

    10. Walker, P.J., Firth, C., Widen, S.G., Blasdell, K.R., Guzman, H., Wood, T.G., Paradkar, P.N., Holmes, E.C., Tesh, R.B., Vasilakis, N., 2015. Evolution of Genome Size and Complexity in the Rhabdoviridae. PLOS Pathogens 11, e1004664.

    11. Walker, P.J., Freitas-Astua, J., Bejerman, N., Blasdell, K.R., Breyta, R., Dietzgen, R.G., Fooks, A.R., Kondo, H., Kurath, G., Kuzmin, I.V., Ramos-Gonzalez, P.L., Shi, M., Stone, D.M., Tesh, R.B., Tordo, N., Vasilakis, N., Whitfield, A.E., ICTV Report Consortium, 2022. ICTV Virus Taxonomy Profile: Rhabdoviridae 2022: This article is part of the ICTV Virus Taxonomy Profiles collection. Journal of General Virology 103, 10.1099/jgv.0.001689.

    12. Zhang, Y.-Z., Chen, Y.-M., Wang, W., Qin, X.-C., Holmes, E.C., 2019. Expanding the RNA Virosphere by Unbiased Metagenomics. Annu. Rev. Virol. 6, 119-139.

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    Genomic characterization of three unclassified rhabdoviruses from mosquitoes in Malaysia and Central Africa

      Corresponding author: Dong-Sheng Luo, dl25703@essex.ac.uk
      Corresponding author: Laurent Dacheux, laurent.dacheux@pasteur.fr
    • a. Institut Pasteur, Université Paris Cité, Unit Lyssavirus Epidemiology and Neuropathology, Paris 75724, France;
    • b. School of Life Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom;
    • c. Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China;
    • d. Department of Infectious Diseases, School of Medicine, Imperial College London, London SW7 2AZ, United Kingdom;
    • e. Institut de Recherche Biomédicale des Armées, Bretigny-sur-Orge 91223, France;
    • f. Institut Pasteur, Université Paris-Cité, National Reference Center for Arboviruses, Paris 75724, France;
    • g. Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou 510005, China

    Abstract: Highlights
    1. Characterisation of three new species of insect-related rhabdoviruses based on coding-complete genome sequences.
    2. Identification of numerous putative genes in addition to the five canonical genes associated with rhabdoviruses.
    3. Demonstration that Porton virus (PORV) and Bangoran virus (BGNV) represent new species within the genus Hapavirus.
    4. Demonstration that Boteke virus (BOTV) is a new species in the genus Sunrhavirus.
    5. Improved knowledge of the genetic diversity of rhabdoviruses, particularly those that are potential arboviruses.

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